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期 刊 ：植物保护学报 2012年

关键词：南方根结线虫；无机盐；孵化；存活；

Keywords:Meloidogyne incognita, inorganic salts, hatch, survival,

摘 要 ：为了明确NH₄HCO₃、CuSO₄·5H₂O、CsCl、NH₄NO₃ 4种无机盐对南方根结线虫卵囊、卵孵化及其幼虫存活的影响。在室内条件下,采用浸渍法测试了4种无机盐在不同浓度和时间处理的作用效果。结果显示:各种无机盐均对线虫卵、卵囊孵化有抑制作用,对2龄幼虫有致死作用。随着无机盐浓度的增加和处理时间的延长,线虫卵、卵囊孵化的抑制率和2龄幼虫的死亡率显著提高。其中0.05 mol/L NH₄HCO₃对线虫卵囊孵化的抑制效果最好,处理16 h达81.17%,24 h后达100%;对线虫卵孵化也有很强的抑制作用,处理16 h达93.33%,24 h后达100%。各种无机盐在不同浓度和处理时间内均对2龄幼虫有致死效果,且半致死中浓度LC₅₀不同,其中CuSO₄·5H₂O的LC₅₀最小,NH₄HCO₃和CsCl次之。表明CuSO₄·5H₂O对南方根结线虫2龄幼虫有较强的致死活性,南方根结线虫2龄幼虫对CsCl的敏感性较高。

Abstract:In order to determine the effects of NH₄HCO₃, CuSO₄·5H₂O, CsCl and NH₄NO₃ on eggs, egg masses hatching and 2nd instar larval (J₂) survival of Meloidogyne incognita, these salts with different concentrations and time were tested in the laboratory using dipping method. The results showed that inorganic salts had strong inhibition to eggs, egg masses hatching and lethal to J₂. The hatching ratio and J₂ mortality were significantly enhanced with the increase of treating concentrations and time. 0.05 mol/L NH₄HCO₃ demonstrated the highest inhibition effect on egg masses hatching with the inhibition ratio 81.17% after 16 h and 100% after 24 h, respectively. 0.05 mol/L NH₄HCO₃ also showed the high inhibition effect to egg hatching with 93.33% inhibition ratio treated after 16 h and 100% after 24 h. Inorganic salts with different concentrations and time had lethal effects to J₂ and had different LC₅₀. CuSO₄·5H₂O showed the smallest LC₅₀, and NH₄HCO₃ and CsCl the second. These results demonstrated that CuSO₄· 5H₂O had the strongest lethal effect to J₂ of M.incognita. And J₂ of M.incognita had the highest sensitivity to CsCl.

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