Abstract:Colorado potato beetle, Leptinotarsa decemlineata Say, is believed to be a disastrous alien invasive pest to China. In order to make a clear understanding of the spatial distribution of Colorado potato beetle in Xinjiang, and to carry out control methods effectively and timely, using random sampling and Iwao regression analysis, spatial distribution pattern, theoretical sampling number of Colorado potato beetle (adults, eggs and larvae), and sequential sampling of larvae were studied through the field investigation, in Urumqi, Xinjiang. The results showed that the spatial distribution patterns of the beetle in each instar were ordinary cluster, and congregation indices were above 1. Sequential sampling was carried on for young-age larvae to determine control index and sampling number. When the control threshold of larvae was 50 heads/quadrat, and the confidence level was 1.96, the upper limit and lower limit control function of sequential sampling was: d1=50n+81 n and d0=50n-81 n. It is necessary to take actions to control Colorado potato beetle when the beetle’s number was higher than 756 heads/100 plants.