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期 刊 ：植物保护学报 2005年

关键词：小菜蛾；抗药性；核型分析；代表性差异分析法；

Keywords:Plutella xylostella (L.), insecticide resistance, karyotype analysis, representational difference analysis (RDA),

摘 要 ：采用核型分析和代表性差异分析方法,探讨了小菜蛾抗药性的分子遗传机理。结果表明,敏感品系和抗性品系小菜蛾的染色体数目均为2n=30条,两者的核型无明显差异;以敏感品系作为驱赶扩增子,抗溴氰菊酯、抗杀虫双、抗杀螟丹近等基因系分别作为检测扩增子进行消减杂交和差异片段的富集。最后随机选取部分差异片段作探针,Southern blot验证,分别在抗杀虫双、抗溴氰菊酯和抗杀螟丹近等基因系中筛选出2、2和3个特异片段。从研究结果可以看出,与敏感品系相比,三种抗性品系小菜蛾的核型没有发生明显的变化,但在DNA水平上存在明显的差异,说明小菜蛾的抗药性与基因组DNA的变化有关。

Abstract:Chromosome analysis and representational difference analysis were conducted on susceptible strain and disultap-resistance and deltamethrin-resistance and cartap-resistance near isogenic strain. The results showed that the number of chromosomal in susceptible and insecticide resistance strain was 2n=30 and the chromosomes present cosh-like. No distinct difference occurred between susceptible and resistance strain. Representational difference analysis (RDA) was applied to find more differences between susceptible and the other three resistant strains. Susceptible strain was used as driver and the other three as testers. Subtractive hybridization and dynamic enrichment were carried out and produced several different fragments. Through southern blot with partial probes we have got 2, 2 and 3 special fragments in the NILS of disultap-resistance, deltmethrin-resistance and cartap-resistance respectively. Compared with the susceptible strain, the resistant strains have no obvious changes in karyotype but have distinct difference in DNA level, which suggests that insecticide resistance of diamondback moth is relative to the genomic DNA changes.

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