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期 刊 ：植物保护学报 2006年

关键词：辣椒疫霉；放线菌；抑菌圈；定殖能力；

Keywords:Phytophthora capsici, actinomycetes, inhibiting zones, colonizing capacity,

摘 要 ：利用抑菌圈 - 定殖力双重测定法进行辣椒疫霉拮抗菌筛选。结果表明:有6株拮抗菌(28G14、03GY02②、F₁、B₂、95W06和31-4G02)在辣椒幼苗根茎部的定殖能力较强(定殖密度大于2.6×10⁴ cfu/g根),其中B₂和F₁的定殖密度为176.0和134.0,分别是致病菌辣椒疫霉(27.0×10⁴ cfu/g根)的6.5倍和5.0倍;这6株拮抗菌均具有较高的相对防效(81.2％~100％),不能定殖的菌株相对防效低(0~37.9％),说明抑菌圈 - 定殖力双重测定法是筛选具有双重功能生防菌的一 种有效而简便的方法; 6株有效菌在灭菌和未灭菌土壤中的相对平均防效分别为93.3％和87.0%;拮抗菌的相对防效与接种时间有关,有效菌株数按十叶期(44株)>开花期(38株)>结椒期(36株)的顺序排列。

Abstract:Biocontrol microorganism against Phytophthora capsici were selected by assessing the zones of inhibition on agar media and root-colonizing capacity, and engaged in biocontrol experiment. The results indicated that: ① The six antimicrobial strains (28G14, 03GY02②, F₁, B₂, 95W06s, 31-4G02) with obvious activity to Phytophthora capsici and the root-colonizing density at pepper roots had higher than 10⁴ cfu/g were screened. The root-colonizing density F₁ and B₂ were 176.0 and 134.0, which were respectively 6.5 and 5.0 times of Phytophthora capsici (27.0×10⁴ cfu/g). ② The six antimicrobial strains colonizing at pepper root had biocontrol efficiency to Phytophthora capsici (81.2%-100%), the strains that could not colonize at hot pepper roots had less effect to Phytophthora capsici (0-37.9%), which elucidated that the method of the zones of inhibition on agar media and root-colonizing capacity were an effective, simple, and convenient method to selected dual function biocontrol microorganism. ③ The six antimicrobial strains colonizing at pepper root had different biocontrol efficiencies in sterilization soil and unsterilized one, which in sterilization soil (93.3%) was higher than that of unsterilized one (87.0%). ④ Biocontrol efficiency of antimicrobial strains involved with inoculating period.

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