摘 要 ：从具典型症状的新鲜百合疫病植株茎基部病组织中分离到百合疫霉菌,根据其病原菌菌丝的形态、菌落特征,厚垣孢子、游动孢子囊和卵孢子的形态和大小,以及病原菌致病性测定,该病原菌鉴定为烟草疫霉Phytophthora nicotianae van Brede de Haan。供试的16种培养基中,病原菌在胡萝卜琼脂培养基(CaA)和辣椒琼脂培养基(PeA)上生长最好,生长速率分别为1.771和1.770mm/h。在常规培养条件下,病原菌不易产生厚垣孢子、游动孢子囊和卵孢子,在低温、皮氏溶液和土壤浸出液中分别诱导产生出大量厚垣孢子、游动孢子囊和卵孢子。
Abstract:The pathogen causing lily blights was isolated from diseased lily with typical symptoms on basal stem. The pathogen was identified as Phytophthora nicotianae van Brede de Haan according to the characteristics of mycelium, chlamydospore, zoosporangium, oospore and the test of pathogenicity of the pathogen. Among 16 kinds of culture media, the best media for pathogen growing and generating were carrot agar medium (CaA) and pepper agar medium (PeA) with growth speed 1.771mm/h and 1.770mm/h respectively. It is hard for Phytophthora nicotianae to produce chlamydospores, zoosporangiums and oospores under normal culture conditions. With the treatment of low temperature, Petri solution and filtered soil suspension, pathogen could generate plenty of chlamydospores, zoosporangiums and oospores.