Abstract:The B biotype of the whitefly, Bemisia tabaci(Gennadius), has invaded many countries and regions of the world in the last 20 years and now become one of the most important crop pests worldwide. One of the major ways causing damage by this pest is transmission of geminiviruses. In this study, the capacity for B biotype of the whitefly to acquire, retain and transmit Tobacco curly shoot virus (TbCSV) was investigated using polymerase chain reaction (PCR). TbCSV DNA was detected in 40% of whiteflies that had fed for 30 min on TbCSV-infected tobacco plants. Percent acquisition of TbCSV by individual whiteflies increased with increase of acquisition time and reached 100% after 12 h. TbCSV DNA was retained in viruliferous whiteflies for about ten days, but not for the entire life of the vector. As shown by the symptoms of the infected plants following virus transmission and the result by PCR, the B biotype of the whitefly proves to be a vector of TbCSV.