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期 刊 ：植物保护学报 2007年

关键词：药材甲；烟草甲；乙酰胆碱酯酶；酶学性质；高浓度二氧化碳气调；

Keywords:Stegobium paniceum, Lasioderma serricorne, AChE, character, CO2 enriched atmosphere,

摘 要 ：用高浓度CO₂气体胁迫处理药材甲Stegobium paniceum和烟草甲Lasioderma serricorne成虫,通过终点比色法测定不同处理时间下两种昆虫乙酰胆碱酯酶AChE的活力变化及其动力学响应,分析高浓度CO₂气调胁迫与AChE性质之间的关系。结果表明,气调处理18h,药材甲和烟草甲比活力分别由对照1.221μmol/mg pro./30min和1.856μmol/mg pro./30min上升到2.231μmol/mg pro./30min 和2.135μmol/mg pro./30min。处理6h内,二者AChE比活力均迅速升高,药材甲AChE在处理9~18h活力上升趋于平缓,而烟草甲AChE活力则在处理6~15h内稍微降低。药材甲和烟草甲分别在处理6 h和9 h内,其AChE动力学参数K_m下降到最低点1.830 μmol/L和7.632μmol/L,随着处理时间延长,其K_m值回升,至18h逐步恢复至对照组水平。高浓度CO₂气调胁迫处理亦导致药材甲和烟草甲AChE V_max的改变。

Abstract:On stress of CO₂ enriched atmospheres, the change of activity of acetylcholinesterase (AChE) in adults of Stegobium paniceum and Lasioderma serricorne and their kinetic indexes were investigated by endpoint method colorimetry. The specific activity of S.paniceum and L.serricorne increased from control 1.221μmol/mg pro./30min and 1.856μmol/mg pro./30min to exposed for 18h 2.231μmol/mg pro./30min and 2.135μmol/mg pro./30min respectively. Increasing specific activity of AChE was noted both insects exposed to CO₂ enriched atmosphere within 6h. There was still slowly growing of specific activity for S.paniceum exposed to the atmosphere for 6 to 18h. However, a gently decreasing trend was noted for L.serricorne exposed to the atmosphere for 6 to 15h. The K_m of AChE got the smallest values(1.830μmol/L and 7.632μmol/L) for S.paniceum and L.serricorne treated within 6 and 9h respectively. But after that, they recovered to normal control pests’ level. Moreover, the V_max of AChE from the two pests was also affected by control atmosphere. Therefore, the conclusion can be drawn that both S.paniceum and L.serricorne can acclimatize themselves to adverse condition (i.e.stress of CO₂ enriched atmosphere) by adjusting of character of AChE.

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