Abstract:Metsulfuron-methyl sprayed with dosages at 30gai/hm2 and 60g ai/hm2 remarkably inhibited acetolactate synthase (ALS) activities in both stems and roots of Alternanthera philoxeroides. Shikimate accumulation was strongly diminished by using glyphosate at dosages of 1537.5g/ hm2 and 3075/ hm2. Meanwhile, the growth rate decrement of Alternanthera philoxeroides was counteracted with the increase of dosages of metsulfuron-methyl and glyphosate. When sprayed glyphosate twice (between 30 days), the inhibition of shikimate content in root tissues was remarkably accelerated by the low glyphosate dosage in comparison with that of the high dosage.