摘 要 ：研究了白花丹Plumbago zeylanica L. 根提取物对柑桔全爪螨的杀螨活性,发现其具有优良的杀螨、杀卵和产卵抑制活性.白花丹根先经甲醇冷浸提取,然后用石油醚萃取甲醇提取物,所得石油醚萃取物的12、24h杀螨致死中浓度(LC50)分别为2.52、2.12mg/ml, 产卵抑制中浓度(ODC50)分别为1.39、1.52mg/ml,杀卵LC50为1.04mg/ml.石油醚萃取物经硅胶柱层析后,以PE5-9馏份的活性最高,1mg/ml浓度下,杀螨率和杀卵率与白花丹素(plumbagin)标样在P=0.05水平均无显著差异.PE5-9馏份 12、24h的杀成螨LC50分别为0.71、0.67mg/ml,杀卵LC50为0.66mg/ml.不同溶剂展开系统薄层层析和GC-MS结果表明,该植物中的杀螨活性主要成分为白花丹素.
Abstract:The acaricidal toxicity to adult mites and eggs,including inhibitory activity to female adult mites,of the root extract of Plumbago zeylanica L.was investigated under laboratory conditions.Root powder of was extracted with methanol at room temperature,the