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作 者 ：江荣风;张福锁

期 刊 ：植物营养与肥料学报 1994年 1卷 1期 页码：67-73

关键词：硼营养；大豆超结瘤突变体；nts382；nod49；结瘤作用；固氮作用；

Keywords:Boron nutrient, Soybean supernodulating ", mutant, nts382, nod49, Nodulation, N2-fixation,

摘 要 ：在短期无氮营养液栽培(28天)条件下,研究缺硼和正常供硼处理对Bragg大豆品种及不结瘤突变体nod49和超结瘤突变体nts382生长、矿质元素吸收与分布及固氮量的影响。试验结果表明:1.在正常供硼的条件下,不结瘤突变体nod的植株生长量,株高、主根生长均高于超结瘤突变体及其亲本。缺硼处理时三种基因型大豆的生长均受到明显的抑制作用。无论是正常供硼还是缺硼处理,超结瘤突变体nts382根系的生长量均明显小于其它基因型。2.正常供硼处理时三种基因型大豆地上部、根及nts382的根瘤中硼的浓度和积累量均高于缺硼处理。缺硼处理,超结瘤突变体及其亲本地上部和根中大部分矿质元素浓度高于正常供硼处理,但积累总量低于正常供硼处理或变化不大。施硼处理的nts382和Bragg,根和根瘤中钙的浓度和积累量明显降低,而地上部变化不大,同时在nts382根瘤中,锌、锰、铁、铜浓度和含量也下降,而钼含量增加。3.施硼处理和Bragg和nts382地上部、根及根瘤中氮的浓度和含量均显著高于缺硼处理,其中超结瘤突变体中氮浓度和总量最高。在缺硼条件下,Bragg和超结瘤突变体能够结瘤,但是Bragg大豆根瘤的固氮作用完全受到抑制,而nts382根瘤还能保持较强的固氮能力。

Abstract:This study was undertaken to detertunins how boron might affect the growth, mineral composition, nodulation and nitrogen fixation in short term hydroponically cultured soybean nodulating mutants (nts382, nod49) and its parent cv. Bragg in N-free solution. The results show that total plant biotnass, plant height and primary root length of nonnqdulating mutant (nod49) were more than those of supernodulating mutant or its parent cv. Bragg in normal boron supply culture (5.0μgmol L-1B) respectively. The root biomass of supernodulating mutant was the lest in three soybean genotypes in both boron treatments. The growth of three soybean genotypes was severely inhibited in non -boron supply treatment. Both nts382 nitrogen concentration and accumulation in each plant part in normal boron supply treatment were more than those in nonboror treatment and nts 382 root nodules exhibited high N₂-fixing capacity in both treatments. Nitrogen fixation of Bragg root nodules was severely inhibited and nts382 was less inhibited and still remained high N₂-fixing capacity in campari-son with Bragg in non-boron culture. Boron supply increased boron concentration and accumulation in each plant part and decreased calcium concentration and accumulation in both root and nodules of Bragg and nts382. Whereas, Mo concentration and accumulation increased in nts 382 nodules but Fe, Mn, Cu, Zn contents decreased in normal boron treatment.

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