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犇犻狏犲狉狊犻狋狔狅犳犅犾犲狋犻犾犾犪狊狋狉犻犪狋犪(犜犺狌狀犫.)犚犮犺犫.犳.
LIJun1,YANGHeng1,ZHOUTianhua1,2
(1ColegeofBiologicalScienceandEngineering,ShaanxiSciTechUniversity,HanzhongShaanxi723001,China;2Qinling
BashanMountainsBioresourcesComprehensiveDevelopmentColaborativeInnovationCenter,Hanzhong,Shaanxi723001,China)
犃犫狊狋狉犪犮狋:犅犾犲狋犻犾犾犪狊狋狉犻犪狋犪(Thunb.)Rchb.f.isanendangeredplantinChinawithimportantmedicinalval
ue.Inthisstudy,thegenomicsequencelibraryandmicrosatelitelibraryof犅.狊狋狉犻犪狋犪wassetupbasedon
Iluminasequencing.SSRprimersweredesignedandcarriedouttotesttheirpolymorphismwith80indi
vidualsfrom4wildpopulations.Fourrelatedspeciesof犅.狊狋狉犻犪狋犪wereusedtoinvestigatedtheircross
taxatransferability.Inaddition,geneticdiversityandgeneticdifferentiationofthespecieswerealsoinves
tigated.Theresultsshowedthat:(1)Microsatelitesequenceswereabundanceinthegenomeof犅.狊狋狉犻犪
狋犪,17841lociweredetectedintotal.100pairofprimersweredesigned,20ofthemperformedhighpoly
morphismafterPCRandpolyacrylamidegelelectrophoresis,thealelesrangedfrom2to6withaverageof
3.85perlocus.Thecrosstaxatransferabilitywasalsoexaminedin4relatedspecies,mostof20lociwere
provedamplifiableinthesespecies.(2)Thestudyrevealedthatthegeneticdiversityof犅.狊狋狉犻犪狋犪was
high(犖a=3.85,犐=1.07,犎=0.6147),geneticdifferentiationwasprominentamongpopulations(犌st=
0.43).Thegeneflowamongpopulationswaslimited(犖m=0.8676),theadjacentpopulationsperformed
tightgeneticrelationshipasrevealedbyclusteranalysesofpopulations.Thereasonsforgeneticconstruc
tionandconservationalstrategiesofthespecieswerealsodiscussedinthisstudy.
犓犲狔狑狅狉犱狊:犅犾犲狋犻犾犾犪狊狋狉犻犪狋犪(Thunb.)Rchb.f.;microsatelite;geneticdiversity;population;primerscreening
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4431 ! " # % & 36_ `1 45:abcde;f@,ghi Table1 Detailedsampleinformationof犅.狊狋狉犻犪狋犪and犅.狊狋狉犻犪狋犪closelyrelatedspeciespopulations È
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ZB !#3Zhenba,Shaanxi 109°26′E,32°06′N 806~1197 20
HY éêg8 Hanyuan,Sichuan 102°26′E,29°39′N 768~1547 20
YX ëh%Yuxi,Yunnan 102°39′E,24°23′N 1426~2105 20 (öij犅.狅犮犺狉犪犮犲犪 WD ëh`¶ Wuding,Yunnan 102°27′E,25°55′N 2019~2311 10 Üij犅.犳狅狉犿狅狊犪狀犪 DL ëhdFDali,Yunnan 100°49′E,25°48′N 1941~2367 10 aij犅.狊犻狀犲狀狊犻狊 MZ ëha) Mengzi,Yunnan 103°03′E,23°24′N 1704~1856 10 )Õ犛.狊犻狀犲狀狊犻狊 LB !&Liuba,Shaanxi 106°52′E,33°37′N 1561~1593 10 b0«®cd , efñÌq¸®ºó 、 a g , xhxi¡¹\9 。 ñòwx{G] ¢"jNOVOGENE6+IklòFDÀ。
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