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Genetic Diversity and Population Structure of Disease Resistance Cotton Cultivars Developed in Shaanxi and Their Descendants by Mapped SSRs

陕棉抗病种质及其衍生品种的遗传多样性与群体结构研究



全 文 :书!"#$%&
,2016,36(8):1551-1559
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犌犲狀犲狋犻犮犇犻狏犲狉狊犻狋狔犪狀犱犘狅狆狌犾犪狋犻狅狀犛狋狉狌犮狋狌狉犲狅犳犇犻狊犲犪狊犲犚犲狊犻狊狋犪狀犮犲
犆狅狋狋狅狀犆狌犾狋犻狏犪狉狊犇犲狏犲犾狅狆犲犱犻狀犛犺犪犪狀狓犻犪狀犱犜犺犲犻狉犇犲狊犮犲狀犱犪狀狋狊
犫狔犕犪狆狆犲犱犛犛犚狊
XINGZenong1,2,JIANGLei2,HEDaohua2,XINGHongyi2,LEIZhongping3
(1BaojiInstituteofAgriculturalSciences,Qishan,Shaanxi722400,China;2ColegeofAgronomy,NorthwestA&FUniversity,
Yangling,Shaanxi712100,China;3ColegeofLifeSciences,NorthwestA&FUniversity,Yangling,Shaanxi712100,China)
犃犫狊狋狉犪犮狋:Fiftyfouraccessions,includingdiseaseresistancecottonlinesdevelopedinShaanxiandtheirde
scendants,weregenotypedtoevaluatetheirgeneticdiversityandpopulationstructureby72SSRmarkers
spanningthewholegenomeofcotton.(1)Thegeneticsimilaritycoefficientof54accessionsrangedfrom
0.7333to0.9872.Among1431similaritycoefficientofpairwiseaccessions,11.1%ofthesimilarityco
efficientwaslessthan0.90,55.6%ofthesimilaritycoefficientwasmorethan0.95.(2)Thepolymorphic
informationcontent(犘犐犆)variedfrom0.04to0.68withanaverageof0.33.(3)Ageneticdistancebased
UPGMAclusteranalysisrevealedthataltheaccessionscouldbedividedintofivegroupsatcutoffvalueof
0.877inthegeneticsimilaritycoefficient.GroupIincluded44accessions,GroupⅢincluded7accessions,
andeachoftheremainedthreegroupsincludedoneaccession,respectively.(4)Amodelbasedclustering
method(implementedbytheprogramSTRUCTURE)showedthataltheaccessionsoriginatedfromfour
clusters.Thisstudyindicatedthatthecottonpopulation,whichwascomprisedofdiseaseresistanceacces
sionsdevelopedinShaanxiandtheirdescendants,wascharacterizedbyhighgeneticsimilaritycoefficient,
narrowgeneticbase(genomicancestry)andpoorgeneticdiversity.
犓犲狔狑狅狉犱狊:cotton;diseaseresistancegermplasm;SSR;geneticdiversity;populationstructure
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Table1 Thepedigreesof54accessions

No
‘’
Accession
/012
Pedigreesource
1 r^2ìBomian2 a^4ì×s^5ìShaanmian4×Xinmian5
2 ^P41 CRI41
{[(5658×a5245)×4067]א10}→^P23→^P41
{[(5658×Shaan5245)×4067]×Zhong10}→CRI23→CRI41
3 7263 Zhong7263 3723×a5012 Zhong3723×Shaan5012
4 ^P40 CRI40 (521א383)א521 (Zhong521×Zhong383)×Zhong521
5 206 Zhong206 (^P43723×a5012)א^P10 (CRI43723×Shaan5012)×CRI10
6 861 a^5ì Ø/LineselectedfromShaanmian5
7 7259 Zhong7259 3723×a5012 Zhong3723×Shaan5012
8 t^56 Chuanmian56 tÍ3ì×a1155 Chuanza3×Shaan1155
9 :63ì Xinluzhong3 (1831×1374)×a401 (1831×1374)×Shaan401
10 u^4ì Yumian4 (vw67×a1155)×(vw67×a40127)(Henan67×Shaan1155)×(Henan67×Shaan40127)
11 ")602 Beinong602 a^4ì×4x58 Shaanmian4×Xuzhou58
12 u^9ì Yumian9 u5×(10א6577) Resistance5×(Zhong10×Zhong6577)
2551 ! " # $ % &                   36y
׈1 ContinuedTable1

No
‘’
Accessions
/012
Pedigreesource
13 tu414Chuankang41 t364×a401 Chuan364×Shaan401
14 z^10Liaomian10 a3215Ø/ LineselectedfromShaan3215
15 6331Zhong6331 (7198×a3215)×{^1ì→5691 (Zhong7198×Shaan3215)×Jimian1→5691
16 {ç321Jihe321 a^4ì×a3765→{757×7523 Shaanmian4×Shaan3765→Ji757×7523
17 a3619 Shaan3619
(5590×|}^)×(4x209×|~^)→F1×a^3ì
(5590×Deltapine)×(Xuzhou209×DeltapineFoster)→F1×Shaanmian3
18 ^48 Yanmian48 a^5ì→861׀^1ì Shaanmian5→861×Lumian1
19 ù^13ì Jinmian13
[(
ù^4ì×a^5ì→79105)×|}^SR1]×{1724
[(Jinmian4×Shaanmian5→79105)×DeltapineSR1]×Ji1724
20 {^11ì Jimian11 ({4ìׂ}4104)→F2×74>102 (Jihan4×Coker4104)→F2×74Yu102
21 {^3ì Jimian3 a^10ìאƒ1ì→a2319 Shanmian10×Zhongchang1→Shaan2319
22 „…^1ìJiangsumian1 a^5ì→861 Shaanmian5→861
23 €1024 Lu1024 c†129×a5245 Shandong129×Shaan5245
24 a5012 Shaan5012 a401Ø/ LineselectedfromShaan401
25 t7327 Chuan7327 a^4ìØ/ LineselectedfromShaanmian4
26 u^1ì Yumian1 a^4ì×<‡1ì Shaanmian4×Liuzhuang1
27 €^N16 Lumianyan16
^P12×(ˆ^1ì×a^9ì→u7675×791→ˆ^3ì)
CRI12×(Simian1×Shaanmian9→Yankang7675×791→Simian3)
28 ‰Š8321Mianyang8321 ‹Œ1ì×a3563 Dongting1×Shaan3563
29 a3215 Shaan3215 a401Ø/ LineselectedfromShaan401
30 u7305 Yankang7305 :^4ì×a^4ì Xinmian4×Shaanmian4
31 „…^3ìJiangsumian3 (a^4ì×<‡1ì→:291)×77369 (Shaanmian4×Liuzhuang1→Xin291)×77369
32 Ž8ìLitai8 ^P7×a^9ì CRI7×Shaanmian9
33 863 a1155×632124 Shaan1155×632124
34 a954 Shaan954 a^6ìØ/ LineselectedfromShaanmian6
35 ^10ì Xiangmian10 (||×a^4ì)×|| (DeltRedDelt×Shaanmian4)×DeltRedDelt
36 864 |}^16×(a^5ì→861) Deltapine16×(Shaanmian5→861)
37 ‘’16 Shiwu16 [u’19×(vw69×a5245)]→u^2ì [Yuwu19×(Henan69×Shaan5245)]→Yumian2
38 {#17 Jizhi17 z632125×(a^10ì×{^1ì) Liao632125×(Shaanmian10×Jimian1)
39 a8092 Shaan8092 z642×a^10ì Liao642×Shaanmian10
40 “1729 Yun1729 |4067×
[(861ה’)×(^P3ìו}^7A)
Deltapine4067×[(861×Beiwu)×(CRI3×Stonevile7A)]
41 …^12 Sumian12 8004×

a^4ì×a^6ì→{757×7523→{ç328)
8004×(Shaanmian4×Shaanmian6→Ji757×7523→Jihe328)
42 u7910 Yu7910 (|}^א^P7ì)×(a^5ì→861) (Deltapine×CRI7)×(Shaanmian5→861)
43 u^16 Yumian16
[(
^P3ì×a^4ì→uu1ì)ז—66]×{^366
[(CRI3×Shaanmian4→Yukang1)×Shangqiu66]×Jimian366
44 a811 Shaan811 z6913×a1155 Liao6913×Shaan1155
45 a401 Shaan401 a^3ì Shaanmian3
46 u^8ì Yumian8 u3478×a1155→u3009×Ôç_˜ Yu3478×Shaan1155→Yu3009×Manymales
47 a3563 Shaan3563 a2021Ø/ LineselectedfromShaan2021
48 ˆ^3ì Simian3 ˆ^1ì×a^9ì→u7675׈Š791Simian1×Shaanmian9→Yankang7675×Siyang791
49 a5245 Shaan5245 a^4ì×a^6ì Shaanmian4×Shaanmian6
50 uu1ì Yukang1 ^P3ì×a^4ì CRI3×Shaanmian4
51 {ç232 Jihe232 ({’303×3016→F2)×3016 (Jiwu303×3016→F2)×3016
52 u^2ì Yumian2 u’19×(vw69×a5245) Yuwu19×(Henan69×Shaan5245)
53 ^P15 CRI15 6429×(a^5ì→861) 6429×(Shaanmian5→861)
54 ù^14 Jinmian14 ™’195×a1155 Fenwu195×Shaan1155
35518š           BC),”:a^uv1.2 e f
1.2.1 5g,hi犇犖犃jk ›!œžŸ,Ë
Ì bp CTAB Ðp!78o DNA[11],)Ì
BeckmanDU800¡(¢k¢¢£þs DNA ¤£
¬¥£ƒ„¦§

;λDNARs¨,Si0.8%©ª
«¬­®¯ƒá°UXy¥£¬w›

1.2.2 犛犛犚 Sl ±ý+²³Ñˆp{|´
0
[1213],
\µjÖ¶€8QJpqr[13]
ØÙ
SSRqr。\26jÖ¶€8ØÙÜ72jqr,o
8·¸¹9$QºÄ»²¼ƒ„B$çà
,SSRk
†PÌ.½o"¾n¿5²¼p-
。PCRÀiÁ
./Ÿ15μL,š78oDNA(20ng/μL)5μL,
10× Buffer(š Mg2+)1.5μL,10mmol/L p
dNTPs0.6 μL,Taq DNA ¢ ç Ã (5U/μL)
0.21μL,ddH2O6.49μL,ÄÀB$(25μmol/L)©
0.6μL。SSR ÀiºqŸ:95 ℃Ŗ3min;
94℃45s,55℃45s,72℃1min,35jÆÇ;
72℃ÈÉ10min;4 ℃ÊË。ÌÍßp+$`
10μLp8~ÎÏÐÔç,95℃–6min,ÑÒ6
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(6%)
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p}˜T™š›
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ò.²¿Ÿ

犘犐犆=1-∑(犘i)2

,犘iˆ¥¨犻j”•78ÜpðÞ。)
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ý

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iÌStructure
2.3.1óô[15],ƒ„7m%ª«p£€/k,ãþ
ñ54t‘’‹ip犙œ(¨ 犻‘’y78o–—2
m¨犽€.pøÞ)。Þ1X€.ù(犓)Ÿ1~
10,ãúX•ûüºýÑp,s72jSSR•ûpk
«ýƒ„k†

§ 犕犆犕犆³ö¦pQRþ(
1Ÿ10000ÿ,!§QRþ(ßp犕犆犕犆1Ÿ
100000ÿ,Nß"ýŒNœLn犘(犇)½ApÉî
Ø!ájç#p犓 œ,ã$Ü7mª«p€.{|
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[16]。
2 ‡`k†
2.1 犛犛犚Vq?BrDSl
-.p72sSSRB$nÌÍÜ176ê}˜
êà

žµsSSRB$ÌÍ2.44ê}˜êà

ˆ2),y;CGR6905、CGR5396`BNL3860½
%

Ÿ1ê}˜êà;;CGR6185`BNL3442½
}

Ÿ4ê}˜êà。íE‰,Ž CGR6185¬
BNL3442pÌÍL&8,;<Š{|}˜²°
(
。SSR•ûp}˜T™š›(犘犐犆))*Ÿ0.04
~0.68Š,ž}˜T™š›Ÿ0.33,y
犘犐犆≥0.6pqrÄ8j,“11%。Áp1+,P
ØÌpSSRB$Žó.d€.}˜T™š›


;<ŠŽï¦§SSR•û8,–—pðÞQ
Ý

{|}~²°¶

G(

ŽNOpiº

S
iÌÍêà` Markerp²°k†Ñ,A}
SSRÌ͟,pƒ£°-,Òü.Ž100~450
bpŠ。
]2 犛犛犚VqstuGvQ?Sw、xyz{|BrD}~€
Table2 DistributionofSSRlociinthechromosomebin,ampliconnumbers,and犘犐犆valueamong54accessions
SSRB$
SSRprimer
Z[.BinNo.
}˜êà
Polymorphic
band
Áêà
Amplicon
}˜
T™š›
犘犐犆
SSRB$
SSRprimer
Z[.BinNo.
}˜êà
Polymorphic
band
Áêà
Amplicon
}˜
T™š›
犘犐犆
BNL2921 Ch01_05 2 2 0.04 CGR6185 Ch17_03 2 3 0.61
CGR5417 Ch01_08 2 2 0.34 CGR6905 Ch17_08 2 3 0.17
BNL3971 Ch02_04 2 2 0.37 BNL3347 Ch19_05 2 3 0.38
CGR5620 Ch03_01 1 1 0.28 BNL3029 Ch19_10 2 2 0.15
CGR5996 Ch03_06 2 2 0.23 CGR5877 Ch19_13 2 2 0.33
BNL3995 Ch05_03 2 2 0.11 CGR5740 Ch20_05 3 4 0.29
CGR5994 Ch05_05 2 3 0.17 CGR5740_1 Ch20_05 2 2 0.08
4551 ! " # $ % &                   36y
׈2 ContinuedTable2
SSRB$
SSRprimer
Z[.BinNo.
}˜êà
Polymorphic
band
Áêà
Amplicon
}˜
T™š›
犘犐犆
SSRB$
SSRprimer
Z[.BinNo.
}˜êà
Polymorphic
band
Áêà
Amplicon
}˜
T™š›
犘犐犆
CGR5083 Ch05_09 2 3 0.24 CGR5740_2 Ch20_05 2 2 0.04
CGR5108 Ch06_02 2 2 0.62 CGR5565a Ch20_07 3 4 0.68
CGR5879 Ch07_01 2 2 0.26 CGR5412 Ch21_01 1 2 0.20
CGR5372 Ch07_05 2 3 0.32 CGR5938 Ch21_02 2 2 0.11
BNL3474 Ch08_06 2 3 0.50 BNL3171 Ch21_03 2 3 0.49
CGR5515 Ch08_09 2 2 0.21 CGR6804 Ch21_05 2 2 0.23
BNL4053 Ch09_01 2 2 0.08 CGR6377 Ch23_04 2 2 0.62
CGR5349 Ch10_01 2 2 0.29 CGR6377 Ch23_04 2 3 0.52
DC20027 Ch10_02 2 2 0.37 CGR5694 Ch23_05 2 2 0.55
BNL2960 Ch10_03 2 3 0.59 BNL3860 Ch24_02 2 3 0.20
BNL3442 Ch11_02 2 3 0.60 CGR6147 Ch24_07 2 3 0.31
CGR5667a Ch05_01,Ch22_04 2 2 0.42 CGR5396 Ch0706,Ch16_11 2 3 0.32
BNL4030 Ch05_03,Ch19_01 2 3 0.54 CGR5565a Ch10_08,Ch20_07 2 2 0.62
BNL3090 Ch05_08,Ch19_10 2 3 0.21 BNL2895 Ch11_06,Ch21_06 2 2 0.21
BNL3594 Ch06_04,Ch25_02 2 4 0.15 CGR6254a Ch12_07,Ch26_07 2 3 0.29
CGR5396 Ch07_06,Ch16_11 2 3 0.04 CGR5576b Ch13_02,Ch17_01 2 2 0.46
CGR5181 Ch07_06,Ch16_11 2 2 0.35 CGR5106 Ch15_07,Ch20_08 2 2 0.58
CGR5796 Ch16_02 2 2 0.24 BNL3103 Ch25_03 2 3 0.53
CGR6083 Ch16_03 2 3 0.65 BNL3103 Ch25_03 2 2 0.24
BNL2986 Ch16_08 2 3 0.49 CGR6932 Ch25_05 2 3 0.17
BNL2986 Ch16_08 2 2 0.61 CGR6679 Ch25_07 2 2 0.18
CGR5729 Ch17_01 2 2 0.34 CGR6880 Ch26_04 2 2 0.11
CGR5670 Ch13_09 2 2 0.18 BNL3871 2 3 0.23
BNL3932 Ch14_02 2 2 0.11 BNL3246 2 2 0.08
BNL3259 Ch14_03 2 3 0.34 BNL3511 2 2 0.24
BNL3644 Ch14_08 2 2 0.37 BNL3778 2 2 0.58
BNL3902 Ch15_03 2 3 0.26 BNL3993 2 2 0.46
CGR5826 Ch15_05 2 2 0.20 BNL4047 2 2 0.15
CGR6129 Ch15_07 2 3 0.32 BNL2896 2 3 0.58
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2 0.391 0.052 0.417 0.14 ClusterC 29 0.049 0.057 0.769 0.125 ClusterC
3 0.418 0.048 0.433 0.101 ClusterC 30 0.241 0.258 0.209 0.292 ClusterD
4 0.029 0.261 0.59 0.119 ClusterC 31 0.046 0.74 0.017 0.196 ClusterB
5 0.046 0.172 0.665 0.117 ClusterC 32 0.01 0.47 0.014 0.506 ClusterD
6 0.247 0.381 0.250 0.122 ClusterB 33 0.008 0.696 0.078 0.218 ClusterB
7 0.028 0.337 0.526 0.108 ClusterC 34 0.005 0.699 0.071 0.225 ClusterB
8 0.044 0.500 0.300 0.156 ClusterB 35 0.049 0.516 0.022 0.413 ClusterB
9 0.401 0.118 0.43 0.051 ClusterC 36 0.018 0.449 0.279 0.253 ClusterB
10 0.313 0.360 0.209 0.118 ClusterB 37 0.015 0.195 0.046 0.744 ClusterD
11 0.019 0.063 0.846 0.072 ClusterC 38 0.975 0.01 0.007 0.007 ClusterA
12 0.247 0.381 0.250 0.122 ClusterB 39 0.966 0.009 0.009 0.016 ClusterA
13 0.031 0.395 0.467 0.106 ClusterC 40 0.826 0.023 0.074 0.077 ClusterA
14 0.43 0.152 0.187 0.231 ClusterA 41 0.946 0.014 0.023 0.017 ClusterA
15 0.013 0.231 0.068 0.687 ClusterD 42 0.749 0.007 0.165 0.078 ClusterA
16 0.052 0.661 0.059 0.228 ClusterB 43 0.649 0.008 0.26 0.083 ClusterA
17 0.514 0.336 0.033 0.118 ClusterA 44 0.02 0.255 0.067 0.658 ClusterD
18 0.121 0.518 0.044 0.316 ClusterB 45 0.293 0.337 0.027 0.343 ClusterD
19 0.009 0.427 0.435 0.129 ClusterC 46 0.023 0.031 0.854 0.093 ClusterC
20 0.855 0.025 0.1 0.02 ClusterA 47 0.5 0.049 0.368 0.083 ClusterA
21 0.048 0.443 0.375 0.134 ClusterB 48 0.012 0.499 0.234 0.255 ClusterB
22 0.05 0.334 0.495 0.121 ClusterC 49 0.012 0.34 0.01 0.639 ClusterD
23 0.015 0.711 0.062 0.212 ClusterB 50 0.034 0.278 0.016 0.672 ClusterD
24 0.012 0.112 0.742 0.134 ClusterC 51 0.011 0.291 0.036 0.662 ClusterD
25 0.014 0.018 0.906 0.063 ClusterC 52 0.444 0.163 0.109 0.285 ClusterA
26 0.016 0.047 0.838 0.099 ClusterC 53 0.063 0.283 0.108 0.546 ClusterD
27 0.076 0.02 0.874 0.031 ClusterC 54 0.126 0.182 0.055 0.637 ClusterD
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