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Phylogeny and Classification of the Moss Family Mielichhoferiaceae in China Inferred from Chloroplast DNA Sequences and Morphology

基于形态学特征和分子数据的中国缺齿藓科初步研究



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,2016,36(7):1366-1375
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6BwrsxjcdG+#$qylez4000{|}~E€,‚ƒ„j…†%6B,‡ˆ‰Šr35‹、40
|Œj4ŽDNA(狉狆狊4、狋狉狀G、狋狉狀L狋狉狀F、犪狋狆B狉犫犮L)‘’“”‰•s–Yks—,˜‰™š›(NJ)qœZ
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(犘狅犺犾犻犪like),×؅ÙÚÛ܅,r®Ý„°ÞÚ,ßÂà…áâã…;OäåæO•çOÔè;édE
êOëdì

Ó¨¬­íîjïðá¼ìédcñ

òëìédcñ
。(4)rcdG+óô¨5È,õcdGÈ
(犕犻犲犾犻犮犺犺狅犳犲狉犻犪)、ÕÖGÈ(犘狅犺犾犻犪)、öÕÖGÈ(犘狊犲狌犱狅狆狅犺犾犻犪)、’dGÈ(犛狔狀狋犺犲狋狅犱狅狀狋犻狌犿)qЯGÈ
(犈狆犻狆狋犲狉狔犵犻狌犿),8÷Eø¿ù34‹(ô‹úseÅo)。
LMN

cdG+

gG+

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se
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犘犺狔犾狅犵犲狀狔犪狀犱犆犾犪狊狊犻犳犻犮犪狋犻狅狀狅犳狋犺犲犕狅狊狊犉犪犿犻犾狔犕犻犲犾犻犮犺犺狅犳犲狉犻犪犮犲犪犲
犻狀犆犺犻狀犪犐狀犳犲狉狉犲犱犳狉狅犿犆犺犾狅狉狅狆犾犪狊狋犇犖犃犛犲狇狌犲狀犮犲狊犪狀犱犕狅狉狆犺狅犾狅犵狔
LIUYongying1,2,NIUYulu2,3,CAOZhen2,ZHAOJiancheng2
(1DepartmentofBiology,JiaozuoTeachersColege,Jiaozuo,Henan454001,China;2ColegeofLifeScience,HebeiNormal
University,Shijiazhuang050024,China;3ColegeofLifeScience,HengshuiUniversity,Hengshui,Hebei053000,China)
犃犫狊狋狉犪犮狋:Thepaperaimstodiscusstherelationshipbetween犕犻犲犾犻犮犺犺狅犳犲狉犻犪groupandBryaceae,toidenti
fymorphologicalsynapomorphybetween犕犻犲犾犻犮犺犺狅犳犲狉犻犪groupandMniaceae,tosolvetheproblemofsys
tematicpositionof犕犻犲犾犻犮犺犺狅犳犲狉犻犪group,tofigureouttheclassificationofMielichhoferiaceaeinChina.
Morphologicalcharactersofmorethan4000specimensofMielichhoferiaceaeanditsrelatedgroupsinChi
nawereanalyzed.Andthen,neighborjoining(NJ)andmaximumlikelihood(ML)treewerebuiltbased
oncombined狋狉狀G,狉狆狊4,狋狉狀L狋狉狀F,and犪狋狆B狉犫犮LcpDNAsequencesof40specimensfor35species.
Combiningmorphologicalandmoleculardataintheanalysesshowedthat:(1)notonlywere犕犻犲犾犻犮犺犺狅犳犲
riagroupandBryaceaefamilyinthedifferentcladesinthemoleculartree,butalsomorphologicalfeatures,
suchastheleafshape,theleafcelsofthetwogroupsweredifferent.犕犻犲犾犻犮犺犺狅犳犲狉犻犪groupshouldbesep
aratedfromBryaceae.(2)Nomorphologicalsynapomorphycanbedetectedfor犕犻犲犾犻犮犺犺狅犳犲狉犻犪groupand
Mniaceaefamily,althoughtheywereinthesamecladeinthemoleculartree.Thetwogroupswerenot
monophyletic.(3)Mielichhoferiaceaeisanaturalmonophyleticgroup.InMielichhoferiaceae,theplants
aresmal,stemstendtobemuchbranchedthaninBryaceae;leavesare犘狅犺犾犻犪likeinshapeandareola
tion;gametangiaareoftenproducedonlateralbranchesorthebaseofstems;peristomeisdiplolepideous
alternate,oftenreducedtoonelayerorrarelyabsent.(4)Therearefivegenera,犕犻犲犾犻犮犺犺狅犳犲狉犻犪,犘狅犺犾犻犪,
犘狊犲狌犱狅狆狅犺犾犻犪,犛狔狀狋犺犲狋狅犱狅狀狋犻狌犿and犈狆犻狆狋犲狉狔犵犻狌犿,inMielichhoferiaceaeinChina,total32speciesand2
variaties.
犓犲狔狑狅狉犱狊:Mielichhoferiaceae;Bryaceae;Mniaceae;morphologicalcharacters;moleculartree;classification
   cdG+(Mielichhoferiaceae)ûȕGü
(Musci),gGýü(Bryidae),gG8(Bryales)。þ
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89E

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se
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犕犻犲犾犻犮犺犺狅犳犲狉犻犪犿犻犲犾犻犮犺犺狅犳犲狉犻犪狀犪,½YZ8p•Ð
]GÈ
(犠犲犻狊狊犻犪)[[G+(Pottiaceae)]r[16]。Nees
2wÅìédqO•çOÔèjOäåE±²MWcdGÈ
(犕犻犲犾犻犮犺犺狅犳犲狉犻犪)[17]。Bruch2]Ec
dGÈjÅìédfÕÖGÈjÇd짨­À
Á

4^_`gG+
(Bryaceae)r[18]。Schimperw
犕犻犲犾犻犮犺犺狅犳犲狉犻犪EabÈM\WcdG+(Mieli
chhoferiaceae),YZcô1ŽÈ[19]。dOj;æT
r

Zæ“se%(
[68,1213,2022]
efBrotherus[4]j
g5

hªgG+ƼŽisjk^‹elæje
z

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édpqrsáïð2m–
ϱ²q#tÐÑ
、¯
×؅
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„°Ú…2u–Ï
±²j¼?ez

cdGÈ

ÕÖGÈ

ЯGÈ
(犈狆犻狆狋犲狉狔犵犻狌犿)、öÕÖGÈ(犘狊犲狌犱狅狆狅犺犾犻犪)、’
dGÈ
(犛狔狀狋犺犲狋狅犱狅狀狋犻狌犿)qvwGÈ(çöx,
犛犮犺犻狕狔犿犲狀犻狌犿)yy8p•¸¹gG+jcdGý
+
(Mielichhoferioideae)、gGý+(Bryoideae)áÕ
ÖGý+
(Pohlioideae)r。
z10{TO,fË{|@¸¹gG+se%6
Bj}`
,¨
lcdG+

gG+qhiG+
(Mni
aceae)jse%~qYklYºÿWP态。
,•„°‚q¯ƒÏDNA„…6B ¡oFºÕ
ÖGÈ

cdGÈqЯGÈ2ezM†•gG+

^fhiG+jlY‡oÌÍ
[23]。
>?g5dO
³8ˆOˆæj‰Š ¡‹ŠŒ
[2426],
‡Ž¼?
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>?çg58Pæ%Káæá‘(
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”Crosby2•
ÕÖGÈ_ÙhiG+

•cdGÈ2p•cdG
+
[1];Hil2[11]–”Koponen[27]l•hiG+js
e%g5

•Goffinet2[28]—˜jóô¨cdGÈ
jZ+
———
hiG+‚™s

sºWcdG+

Frey2[14]@Gofinet2jGeseYk[28]¤+$w
úÅošW›œ

wÕÖGÈ

öÕÖGÈ

cdG
È

’dGÈqvwGÈM\WcdGý+

ЯG
È8p•hiGý+
(Mnioideae)r。
l•rcdG+j6B

žŸ
[6]
¤

rG
eÈ 
》(
®¡

rhªWBrotherus[4]jg5,•c
dGÈr¢¼j‹
———
r£cdG
(犕犻犲犾犻犮犺犺狅犳犲
狉犻犪狊犻狀犲狀狊犻狊)p•gG+cdGý+Ir,fdº¤
jr(CFG 
[2935]
-rFG#$6Bj4
S¥¦

rFG 
》(
§4¨)[12]q;¥¤(犕狅狊狊
犉犾狅狉犪狅犳犆犺犻狀犪)[13]©ª‰žŸjGeseY
k
[6],
•cdGezp•gG+r

‡«¬vez¤
r¨4È33‹(ô‹úseÅo)。NÉ,ÕÖG
Èç[xW3‹:­¯ÕÖG(犘狅犺犾犻犪犿犪犮狉狅犮犪狉
狆犪)[36]、£dÕÖG(犘.犺狔犪犾狅狆犲狉犻狊狋狅犿犪)[36]q®¯
ÕÖG
(犘.犺犻狊犪犲)[37];œz,9:;2[xW’dG
È1ç‹———°±’dG(犛狔狀狋犺犲狋狅犱狅狀狋犻狌犿犽狌狀
犾狌狀犲狀狊犲),vÈÆrj²³«¬[38]。þ®‹V,c
dGez¤r´«¬#¨5È34‹(ô‹úse
Åo
)。
µ¶·2
[39]
,•‚¸ÏDNAITS„…j
Ykpq猔s—]E

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ƼŽ»\•hiG+I¼jez

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M\cdG+jg5

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[4041]
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Bvez}~

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?‹j[x
。《
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》[15]
r
ÂÃFrey2[14]Ge#$seYk•cdG2ez
p•hiG+r

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ÄÅFG#$ 
》[42]
r³˜‰=Yk

~¥Æ¤H¾,•…†%±²qs–“”j6
B

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•@Ge#$gG8jYkse6B§¨4S˹

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1.1 U V
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j}~4000{|。ˆ‰40|Œ,¿35‹‰•
s–O$%‰Š

ŠrhiG+7ŽŒ,ÕÖGÈ
14ŽŒ,ÌÍGÈ(犅狉犪犮犺狔犿犲狀犻狌犿)7ŽŒ,g
GÈ7ŽŒ,ÎGÈ(犃狀狅犿狅犫狉狔狌犿)、+éGÈ
(犘犾犪犵犻狅犫狉狔狌犿)、Z¯GÈ(犚犺狅犱狅犫狉狔狌犿)qÏÐG
È
(犔犲狆狋狅犫狉狔狌犿)"1ŽŒ,ÑÒGÚÓԋ
(犌狔犿狀狅狊狋狅犿犻犲犾犾犪狏犲狉狀犻犮狅狊犪var.犪犮狌犿犻狀犪狋犪)E¼e
z

€ƒ„IÕÖ¢2。
W1 BCDEXYPZ;P[\]^_`W
Table1 TaxonomicsystemofMielichhoferiaceae
‹È+
Family
ý+×s
Subfamily
‹ôÈ
Genus
¥¦
References
gG+Bryaceae
cdGý+Mielichhoferioideae cdGÈ犕犻犲犾犻犮犺犺狅犳犲狉犻犪,ØÙGÈ(çöx)犎犪狆犾狅犱狅狀狋犻狌犿
gGý+Bryoideae ÕÖGÈ犘狅犺犾犻犪,¸ÚGÈ犕狀犻犫狉狔狌犿,ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿
[3]
gG+Bryaceae
cdGý+Mielichhoferioideae ’dGÈ 犛狔狀狋犺犲狋狅犱狅狀狋犻狌犿

ØÙGÈ 犎犪狆犾狅犱狅狀狋犻狌犿,cdG
È犕犻犲犾犻犮犺犺狅犳犲狉犻犪
[4]
gGý+Bryoideae
ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿,¸ÚGÈ犕狀犻狅犫狉狔狌犿,ÕÖGÈ犘狅犺犾犻犪,
öÕÖGÈ犘狊犲狌犱狅狆狅犺犾犻犪
gG+Bryaceae ÕÖGý+Pohlioideae cdGÈ犕犻犲犾犻犮犺犺狅犳犲狉犻犪,ÕÖGÈ犘狅犺犾犻犪 [5]
gG+Bryaceae
cdGý+Mielichhoferioideae ’dGÈ 犛狔狀狋犺犲狋狅犱狅狀狋犻狌犿

ØÙGÈ 犎犪狆犾狅犱狅狀狋犻狌犿,cdG
È犕犻犲犾犻犮犺犺狅犳犲狉犻犪
[6]
gGý+Bryoideae
ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿,¸ÚGÈ犕狀犻狅犫狉狔狌犿,ÕÖGÈ犘狅犺犾犻犪,
öÕÖGÈ犘狊犲狌犱狅狆狅犺犾犻犪
gG+Bryaceae ÕÖGý+Pohlioideae
cdGÈ犕犻犲犾犻犮犺犺狅犳犲狉犻犪,ÕÖGÈ犘狅犺犾犻犪,ЯGÈ犈狆犻狆狋犲狉狔犵犻
狌犿,¸ÚGÈ犕狀犻狅犫狉狔狌犿
[7]
gG+Bryaceae
cdGý+Mielichhoferioideae cdGÈ犕犻犲犾犻犮犺犺狅犳犲狉犻犪 [8]
gGý+Bryoideae ÕÖGÈ犘狅犺犾犻犪
cdG+ Mielichhoferiaceae ——— cdGÈ 犕犻犲犾犻犮犺犺狅犳犲狉犻犪

öÕÖGÈ 犘狊犲狌犱狅狆狅犺犾犻犪,vwGÈ
犛犮犺犻狕狔犿犲狀犻狌犿,’dGÈ犛狔狀狋犺犲狋狅犱狅狀狋犻狌犿
[1]
hiG+ Mniaceae ——— ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿,ÕÖGÈ犘狅犺犾犻犪
gG+Bryaceae ——— cdGÈ犕犻犲犾犻犮犺犺狅犳犲狉犻犪,ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿 [9]
hiG+ Mniaceae ——— ÕÖGÈ犘狅犺犾犻犪
hiG+ Mniaceae ÕÖGý+Pohlioideae ÕÖGÈ犘狅犺犾犻犪,cdGÈ犕犻犲犾犻犮犺犺狅犳犲狉犻犪,ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿 [10]
cdG+ Mielichhoferiaceae ——— ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿

ÕÖGÈ犘狅犺犾犻犪,vwGÈ犛犮犺犻狕狔犿犲狀犻
狌犿,cdGÈ犕犻犲犾犻犮犺犺狅犳犲狉犻犪
[11]
gG+Bryaceae ———
cdGÈ犕犻犲犾犻犮犺犺狅犳犲狉犻犪,ÕÖGÈ犘狅犺犾犻犪,ЯGÈ犈狆犻狆狋犲狉狔犵犻
狌犿,öÕÖGÈ犘狊犲狌犱狅狆狅犺犾犻犪
[12,13]
gG+Bryaceae ——— ØÙGÈ犎犪狆犾狅犱狅狀狋犻狌犿 [14]
hiG+ Mniaceae
cdGý+Mielichhoferioideae cdGÈ 犕犻犲犾犻犮犺犺狅犳犲狉犻犪

öÕÖGÈ 犘狊犲狌犱狅狆狅犺犾犻犪,ÕÖGÈ
犘狅犺犾犻犪,vwGÈ犛犮犺犻狕狔犿犲狀犻狌犿,’dGÈ犛狔狀狋犺犲狋狅犱狅狀狋犻狌犿
hiGý+ Mnioideae ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿
cdG+ Mielichhoferiaceae ——— cdGÈ 犕犻犲犾犻犮犺犺狅犳犲狉犻犪

öÕÖGÈ 犘狊犲狌犱狅狆狅犺犾犻犪,ÕÖGÈ
犘狅犺犾犻犪,ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿
[2]
hiG+ Mniaceae ——— cdGÈ 犕犻犲犾犻犮犺犺狅犳犲狉犻犪

öÕÖGÈ 犘狊犲狌犱狅狆狅犺犾犻犪,ÕÖGÈ
犘狅犺犾犻犪,ЯGÈ犈狆犻狆狋犲狉狔犵犻狌犿
[15]
8631 ! " # $ % &                   36¨
W2 abcdefg犌犲狀犅犪狀犽hi$
Table2 TaxasequencedinthisstudywithGenBankaccessionnumbersandvoucherspecimeninformation
seTaxon
+Family ‹Species
ی}~
Voucherinformation
GenBankܬÝGenBankaccessionnumber
狉狆狊4 狋狉狀G 狋狉狀L狋狉狀F 犪狋狆B狉犫犮L
cdG+
Mielichho
feriaceae
ÞÔÕÖG犘狅犺犾犻犪犾犪狋犻犮狌狊狆犻狊 LMNZhaoJiancheng20072654 KX355967 KX289674 — KX355896
ÕÖG犘狅犺犾犻犪犲犾狅狀犵犪狋犪 ßßh£·àáâSulaymanMamtimin07100 KX355968 KX289675 KX355934 KX355897
¶ãÕÖG犘狅犺犾犻犪犾犲狌犮狅狊狋狅犿犪 µäå WangMeizhi081085 KX355985 KX289676 — KX355898
æÕÖG犘狅犺犾犻犪狀狌狋犪狀狊 ßßh£·àáâSulaymanMamtimin14985 KX355971 KX289677 KX355935 KX355899
öÚéÕÖG犘狅犺犾犻犪犾狅狀犵犻犮狅犾犾犻狊 ßßh£·àáâSulaymanMamtimin200426 KX355972 KX289678 KX355936 KX355900
/ýÕÖG犘狅犺犾犻犪犵犲犱犲犪狀犪 1çGaoQian18376 KX355973 KX289679 KX355937 KX355901
èéÕÖG犘狅犺犾犻犪狆狉狅犾犻犵犲狉犪 é­CaoTong93277 KX355986 KX289680 — KX355902
èéÕÖG犘狅犺犾犻犪狆狉狅犾犻犵犲狉犪 êëìLiLiangqian764 KX355987 KX289681 KX355938 KX355903
kOÕÖG犘狅犺犾犻犪犮狉狌犱犪 í HeQiang838 KX355975 KX289682 KX355939 KX355904
öÚéÕÖG犘狅犺犾犻犪犾狅狀犵犻犮狅犾犾犻狊 í HeQiang718 KX355976 KX289683 KX355940 KX355905
kOÕÖG犘狅犺犾犻犪犮狉狌犱犪 í HeQiang698 KX355977 KX289684 JF277360.1 KX355906
ÕÖGæ†Ô‹犘狅犺犾犻犪犲犾狅狀犵犪狋犪var.犿犻狀狅狉 îï;PeiLinying192 KX355969 KX289685 KX355941 KX355907
/ýÕÖG犘狅犺犾犻犪犵犲犱犲犪狀犪 êðñLiFenxia1053 KX355970 KX289686 KX355942 KX355908
öÚéÕÖG犘狅犺犾犻犪犾狅狀犵犻犮狅犾犾犻狊 9:;LiuYongying1158 KX355974 — JF277344.1 KX355909
hiG+
Mniaceae
Ó¯òiG犘犾犪犵犻狅犿狀犻狌犿犮狌狊狆犻犱犪狋狌犿 ó£ôXiaoMingxuan0417 KX355980 KX289688 KX355944 KX355911
Ÿ…ÑiG犜狉犪犮犺狔犮狔狊狋犻狊狌狊狊狌狉犻犲狀狊犻狊 ó£ôXiaoMingxuan0087 KX355984 KX289689 KX355945 KX355912
Ÿ…ÑiG犜狉犪犮犺狔犮狔狊狋犻狊狌狊狊狌狉犻犲狀狊犻狊 ó£ôXiaoMingxuan0086 KX355981 KX289690 KX355946 —
Z¯õiG犘犾犪犵犻狅犿狀犻狌犿狊狌犮犮狌犾犲狀狋狌犿 ó£ôXiaoMingxuan0420 KX355978 KX289691 KX355947 KX355913
ö¯hiG犕狀犻狌犿狊狆犻狀狅狊狌犿 ó£ôXiaoMingxuan0098 KX355983 — KX355948 KX355914
÷¯hiG犕狀犻狌犿狋犺狅犿狊狅狀犻犻 ó£ôXiaoMingxuan0415 KX355982 KX289692 KX355949 KX355915
øùõiG犘犾犪犵犻狅犿狀犻狌犿犲犾犾犻狆狋犻犮狌犿 ó£ôXiaoMingxuan0105 KX355979 KX289693 KX355950 KX355916
gG+
Bryaceae
ú¯+éG犘犾犪犵犻狅犫狉狔狌犿犵犻狉犪犾犱犻犻 ûüý WangYoufang4354A KX355958 KX289687 KX355943 KX355910
ÎG犃狀狅犿狅犫狉狔狌犿犼狌犾犪犮犲狌犿 9:;LiuYongying5036 KX355951 AY163052.1 KX355917 KX355880
[OÌÍG犅狉犪犮犺狔犿犲狀犻狌犿狆犲狀犱狌犾狌犿 ßßh£·àáâSulaymanMamtimin07021 KX355952 KX289659 KX355918 KX355881
®ÔÌÍG犅狉犪犮犺狔犿犲狀犻狌犿犲狓犻犾犲 êþÿLiXiuqin99063 KX355961 KX289660 KX355919 KX355882
!ùÌÍG犅狉犪犮犺狔犿犲狀犻狌犿犾狅狀犵犻犱犲狀狊 é"CaoNa20070929 KX355965AY163055.1 KX355920 KX355883
#éÌÍG犅狉犪犮犺狔犿犲狀犻狌犿狆狋狔犮犺狅狋犺犲犮犻狌犿 û$£ WangQinghua751 KX355953AY163058.1 KX355921 KX355884
j¯ÌÍG犅狉犪犮犺狔犿犲狀犻狌犿犮犪狆犻狋狌犾犪狋狌犿 êðñLiFenxia1633 KX355962 KX289661 KX355922 KX355885
r£ÌÍG犅狉犪犮犺狔犿犲狀犻狌犿狊犻狀犲狀狊犲 µäå WangMeizhi48304 KX355955 KX289662 KX355923 KX355886
ÌÍG犅狉犪犮犺狔犿犲狀犻狌犿狀犲狆犪犾犲狀狊犲 é"CaoNa20070948 KX355966 KX289663 KX355924 AF546823.1
æ%gG犅狉狔狌犿狆犪犾犾犲狊犮犲狀狊 ßßh£·àáâSulaymanMamtimin15265 KX355957 KX289666 KX355926 KX355889
„¯gG犅狉狔狌犿犮犪狆犻犾犾犪狉犲 &(NiuYulu20100196 KX355956 KX289667 KX355927 KX355890
&égG犅狉狔狌犿狋狌狉犫犻狀犪狋狌犿 ßßh£·àáâSulaymanMamtimin15095 KX355960 KX289668 KX355928 KX355891
)%gG犅狉狔狌犿狆狌狉狆狌狉犪狊犮犲狀狊 &(NiuYulu110045 KX355959 KX289669 KX355929 KX355892
ú…gG犅狉狔狌犿犪犿犫犾狔狅犱狅狀 *¦+PengXuanjun20050189 — KX289670 KX355930 KX355893
¨¯gG犅狉狔狌犿狋犺狅犿狊狅狀犻犻 &(NiuYulu20110900 KX355954 KX289671 KX355931 KX355894
,–gG犅狉狔狌犿犫狅狉狀犺狅犾犿犲狀狊犲 9:;LiuYongying415 KX355963 KX289672 KX355932 KX355895
Z¯G犚犺狅犱狅犫狉狔狌犿狉狅狊犲狌犿 ûüý WangYoufang2011-0127 KX355964 KX289673 KX355933 —
-G+
Meesiaceae ÏÐG犔犲狆狋狅犫狉狔狌犿狆狔狉犻犳狅狉犿犲 ê.LiLin030570 KX355988 KX289665 AF023736.1 KX355888
[G+
Pottiaceae
ÑÒGӯԋ犌狔犿狀狅狊狋狅犿犻犲犾犾犪狏犲狉狀犻犮狅
狊犪var.犪犮狌犿犻狀犪狋犪 9:;LiuYongying05433 KX355989 KX289664 KX355925 KX355887
/
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Note:indicatessequencedownloadfromGenBank,and—indicatesabsentdata
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‰DNAhABCD("EF–Oð+G¨HIJ)
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Y

N DNA20ng,dNTP200mmol·L-1,10×
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ÔÁ7min,œd72℃TU5min。rÉ35ŽV
W

ºR”ú
:狉狆狊4、狋狉狀L狋狉狀F:95℃(25s),58℃
(5s),72 ℃ (2 min);犪狋狆B狉犫犮L、狋狉狀G:95 ℃
(25s),47℃(5s),72℃(2min)。OÊX$Y®Z
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‹^„…h_
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(http://blast.ncbi.nlm.nih.gov/Blast.cgi)f
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žMYkŸ

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‚æ„…’‡“”tYks—

~6Brjse
zóXWhiG+

gG+

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(Meeseaceae)2;S•‰ŠrÝs}~T!LMu
v‰•s–Yks—

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狋狉狀Fq犪狋狆B狉犫犮L24Ž„…jŒw¨40|。@
W3 tufg:vw
Table3 Informationofprimersusedtoamplify
thefourregions
DNA
Marker
“$„…
Primer
ÂÁ¥¦
Reference
狉狆狊4 R
:TACCGAGGGTTCGAATC
F:ATGTCCCGTTATCGAGGACCT
[43]
犪狋狆B狉犫犮L R
:ACATCKARTACKGGACCAATAA
F:AACACCAGCTTTRAATCCAA
[44]
狋狉狀L狋狉狀F R
:CGAAATCGGTAGACGCTACG
F:ATTTGAACTGGTGACACGAG
[45]
狋狉狀G R
:ACCCGCATCGTTAGCTTG
F:GCGGGTATAGTTTAGTGG
[46]
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Mx„…js—@‰™š›
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~jXO

T^žMºy€jYkpqŸ
[49]。
2  ¡fs—
NJq MLj‹s—C›@4ŽDNA‘
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(犘犾犪犵犻狅犿狀犻狌犿)qÑiGÈ(犜狉犪犮犺狔犮狔狊狋犻狊)3
N

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(犘狅犺犾犻犪犮狉狌犱犪)、3|öÚéÕÖG(犘.犾狅狀犵犻
犮狅犾犾犻狊)、ÞÔÕÖG(犘.犾犪狋犻犮狌狊狆犻狊)、2|/ýÕÖ
G
(犘.犵犲犱犲犪狀犪)、ÕÖG(犘.犲犾狅狀犵犪狋犪)qæÕÖG
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Numbersabovebranchesarebootstrapvalues≥50%from1000replicates.A.犘狅犺犾犻犪Mniaceae;B.Bryaceae;
a.Section犘狅犺犾犻犪;b.Section犆犪犮狅犱狅狀;c.Mniaceae
Fig.1 Consensustreeofneighborjoining(NJ)treederivedfrom狉狆狊4+狋狉狀G+狋狉狀L狋狉狀F+犪狋狆B狉犫犮Ldata
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C,H,J.Distalleafcels;D,G,I.Medialleafcels
Fig.2 ComparisonofmorphologicalcharactersforMielichhoferiaceae,BryaceaeandMniaceae
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