全 文 :书!"#$%&
,2016,36(7):1285-1293
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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犆犾狅狀犻狀犵犪狀犱犉狌狀犮狋犻狅狀犪犾犃狀犪犾狔狊犻狊狅犳犆犻犖犃犆1犳狉狅犿犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪
YUEJunyan,YUEWenran,YANGQi,YUXiumin,YANGFeiyun,
WANGGuangxia,LIGuojing,WANGRuigang,CONGJingyu
(InnerMongoliaAgriculturalUniversity,Hohhot010018,China)
犃犫狊狋狉犪犮狋:NACsareoneofthelargestplantspecifictranscriptionfactorfamilies.Membersinthisfamily
playimportantrolesingrowthandabioticstressresponses.Inthisstudy,thefullengthcDNAsequence
of犆犻犖犃犆1from犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪wasclonedbyPCRtechnique.Itwas1066bpinlength.Bioinfor
maticsanalysisshowedthat犆犻犖犃犆1hadanopenreadingframeof921bp,encoding306aminoacids.The
calculatedmolecularweightofCiNAC1was34.57kDandtheisoionicpointwas8.35.TheCiNAC1pro
teinwasahydrophilicproteinwhichhadaconservedNAMdomainintheNterminus.Itcontained26
phosphorylationsitesand7glycosylationsites.RealtimequantitativePCRanalysisshowedthat犆犻犖犃犆1
wasinducedbydrought,NaCl,dehydration,andhighpH.LocalizationassaysrevealedthatCiNAC1lo
calizedinthenuclei,consistentwithitsroleastranscriptionfactor.Overexpressionof犆犻犖犃犆1promoted
thelateralrootformationandlengthoftheprimaryroot.Theseresultsindicatedthat犆犻犖犃犆1mightbe
relatedtoresponsetoenvironmentalstressin犆.犻狀狋犲狉犿犲犱犻犪.
犓犲狔狑狅狉犱狊:犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪;NACtranscriptionfactors;abioticstress;functionalanalysis
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1.2.6 jkeflm狆犆犪狀犌犆犻犖犃犆1n狆犆犃犕
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(ORF)921bp,Ø306(2)。
2.2 犆犻犖犃犆1Ya犖犃犆K
@tCD
p,DNAMAN5.2£«#CiNAC1
ÄÇÈÉ ANAC019、 MtNAC1、` B Gm
NAC35、ÒK`BGaNAC21/22lù!
1 犆犻犖犃犆1gcDNAHn
M.DL5000 1.犆犻犖犃犆1
Fig.1 Amplificationof犆犻犖犃犆1cDNA
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3)¶ÕCiNAC1ÄW
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CiNAC1Ä
NAC1(犕犲犱犻犮犪犵狅狋狉狌狀犮犪狋狌犾犪)、¹
(犃犿
犿狅狆犻狆狋犪狀狋犺狌狊犿狅狀犵狅犾犻犮狌狊)、` B NAC35(犌犾狔犮犻狀犲
犿犪狓)、ÒK`BNAC21/22(犌犾狔犮犻狀犲狊狅犼犪)、"
(犕犪犾狌狊犺狌狆犲犺犲狀狊犻狊)、ØØL NAC21/22(犜犺犲狅
犫狉狅犿犪犮犪犮犪狅)、pNAC40(犕犪狀犻犺狅狋犲狊犮狌犾犲狀狋犪)、
KNAC1(犘狅狆狌犾狌狊狋狉犻犮犺狅犮犪狉狆犪)、K×
KNAC1(犘狅狆狌犾狌狊狋狉犲犿狌犾犪×犘狅狆狌犾狌狊犪犾犫犪)、
L
(犆犪犿犲犾犾犻犪狊犻狀犲狀狊犻狊)、ÇÈÉ NAC1(犃狉犪犫犻犱狅狆狊犻狊
狋犺犪犾犻犪狀犪)ù!Ë\ù¬LP,¨ (4)¶Õy
CiNAC1ÄMtNAC1Go,Å
l-£·89%,¡ÞËm2。
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2 犆犻犖犃犆1gcDNAu;
TheATGinitiationcodonisunderlined;Theterminalcodonisunderlinedwithasterisk
Fig.2 ThecDNAandthededucedaminoacidsequenceof犆犻犖犃犆1
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(NP_175697.1);CiNAC1.y
;MtNAC1.(XP_003595973.1);GmNAC35.`B(NP_001235901.1);GsNAC21/22.ÒK`B(KHN26800.1)
3 CiNAC1ÄW^NAC4¦§¨©ªlÖ0
TheunderlinedsectionsarefiveconservativesubdomainsAtoEinNAC.Aminoacidresiduesconservedinalsequencesareboxed
inblack,whilesimilaraminoacidsareboxedingray.AlabbreviationandGenBankAccessionNo.areshownas:ANAC019.
犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪(NP_175697.1);CiNAC1.犆犪狉犪犵犪狀犪犻狀狋犲狉犿犲犱犻犪;MtNAC1.犕犲犱犻犮犪犵狅狋狉狌狀犮犪狋狌犾犪(XP_003595973.1);
GmNAC35.犌犾狔犮犻狀犲犿犪狓 (NP_001235901.1);GsNAC21/22.犌犾狔犮犻狀犲狊狅犼犪(KHN26800.1)
Fig.3 AlignmentofNACdomainbetweenCiNAC1andotherNACs
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;
P¨2lΩ¶BootstrapêyÑ1000kze}«lØSïl¬PÖ;®¶¯¬°±
4 CiNAC1ÄW^$ NACslË\ù¬P
TheaccessionNo.wasgiveninbracket;Thenumbersonthebranchesrepresentthereliabilitypercentofbootstrapvaluesbased
on1000replications;Scaleplaterepresentstheevolutiondistanceoftheseplants
Fig.4 PhylogeneicanalysisofCiNAC1andNACsfromotherspecies
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ûlNAM¦§¨©ª。g_,Øë²by
犆犻犖犃犆1g³ÑNAChi。
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NAC1l«¬b®¬°vÁ,
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32、48、121、124、226、269)b26«¬,W
y26«¬äg(Thr)«¬
(50、82、112、161、199、236、267、268、334、341、379、
395、429、453、455、466、485、559、584、670、686、736、
737、791、877、890)。
2.3.3 犆犻犖犃犆1=j= K,
SOPMAGX£«MµCiNAC1lZ[¨©,
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fCD
ùoË12犆犻犖犃犆1gl{|,¯Ã
¨, qRTPCR 5~´µy
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NaCl、¼¢、»,¹º、-pH"%ëì犆犻犖犃犆1
g¶·lP¬
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犆犻犖犃犆1transgeniclines
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:
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