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作 者 ：赵 胡

期 刊 ：西北植物学报 2012年 9期 页码：1726~1730

关键词：阜豆；ABC转运蛋白；基因克隆；表达分析；

Keywords:Fuyang soybean, ABC transporter, gene isolation, expression analysis,

摘 要 ：利用RACE技术从大豆品种‘阜豆11号’中克隆到1个ABC转运蛋白基因,该基因cDNA全长为4 693 bp,其中开放读码框4 341 bp,编码1 447个氨基酸,分子量162.5 kD,具有高度保守的ATP结合位点,命名为GmABC。 蛋白序列比对结果显示，该基因编码蛋白属于PDR(pleiotropic drug resistance) 多向耐药性蛋白家族成员。系统进化树分析显示，GmABC与苜蓿PDR亲缘关系最近,相似性达84%。基因表达分析显示，GmABC受镉诱导表达,当Cd²⁺浓度达到100 mg·L^-1时,其表达量最高。研究表明，GmABC可能对阜豆镉胁迫抗性发挥重要作用。

Abstract:In this study,a member of ABC transporter gene named GmABC,was isolated from Fuyang soybean ( Fuyang Glycine max NO.11) using RACE (rapid amplification of cDNA ends) method.The full length of cDNA was 4 693 bp with a 4 341 bp open reading frame (ORF) which encoded a peptide of 1 447 amino acids and containing the highly conserved ATP binding site modules,and the predicted protein molecular mass was 162.5 kD.The protein sequence alignment showed that this gene encoded a protein belonging to the PDR (pleiotropic drug resistance) family of ABC proteins.The phylogenetic analysis showed that GmABC has the highest similarity with PDR from Medicago truncatula,which shared 84% homology with Medicago truncatula in the amino acid sequence.Expression analysis indicated that GmABC gene was induced by Cd²⁺,expression level of GmABC gene was the highest at Cd²⁺ concentration of 100 mg·L^-1.The results suggested that GmABC may play an important role in Cd stress resistance of Fuyang soybean.

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