---

作 者 ：袁丽环1，王 甜2，王文科1*

期 刊 ：西北植物学报 2012年 5期 页码：956~962

关键词：苦荞；活性炭；根系生长；碳氮代谢；保护酶；

Keywords:Tartary buckwheat, activated charcoal, roots growth, C and N metabolism, protective enzyme,

摘 要 ：以山西省主栽苦荞品种‘黑丰1号’温室盆栽幼苗为材料，设置土壤活性炭含量分别为0（CK）、2.5（B_2.5）、5.0（B_5.0）、7.5（B_7.5）、10（B₁₀） g/kg共5个水平，研究土壤中施加活性炭后对苦荞幼苗根系及碳氮代谢、保护酶活性等指标的影响。结果显示：(1)随着活性炭施用比例的增加，苦荞幼苗根系生长指标和根系活力指标均呈先增后减的趋势，根平均直径呈先减后增的趋势，其中B_5.0、B_7.5处理的幼苗根系总长度、总表面积、总体积、活跃吸收面积、根尖数均显著高于对照，但B₁₀处理的根系发育减弱。(2)随活性炭施用比例的增加，苦荞幼苗叶片蔗糖酶活性变化呈先增后减的趋势，同一处理水平条件下随苦荞的生长而逐渐下降；B_2.5、B_5.0处理苦荞幼苗叶片蔗糖酶活性和可溶性糖含量均比CK极显著增加，B_7.5处理略有提高，B₁₀处理差异不显著。(3)苦荞幼苗叶片谷氨酰胺合成酶（GS）活性随活性炭的增加基本呈上升趋势，而同一处理水平下随苦荞的生长而下降；叶片GS活性在B_5.0、B_7.5处理时比CK极显著提高，可溶性蛋白质含量在B_7.5处理时也显著提高。(4)叶片保护酶SOD、POD、CAT活性随活性炭浓度的升高呈先升后降的变化趋势，而同一处理水平下各时期间变化不大；B_2.5处理叶片的SOD、POD和CAT活性比对照显著增强。研究发现，适量施用活性炭（2.5～7.5 g/kg）能有效促进苦荞幼苗碳氮代谢和保护酶活性，增强其根系活力。

Abstract:In order to study the effects of activated carbon on the roots growth,C and N metabolism and protective enzymes of ‘Heifeng No.1’ in Shanxi,various doses of activated carbon(0,2.5,5.0,7.5 and 10 g/kg) were added into soil in greenhouse pot.Results showed that:(1)With the concentration of activated carbon increasing,the roots growth showed an optimal performance and the average diameter showed a minimum value;Total root length,total surface areas,total volume,root active absorbing areas and the number were improved significantly (P<0.05) with 5.0 (B_5.0) and 7.5 (B_7.5) g/kg activated carbon applied,but the root grew weak with 10 g/kg activated carbon(B₁₀).(2)With the concentration of activated carbon increasing,the activity of invertase reached a peak at B_5.0,and then decreased gradually in pot culture;Invertase activity and soluble sugar content were increased significantly (P<0.01) treated by 2.5 (B_2.5) and 5.0 (B_5.0) g/kg activated carbon,and B_7.5 and B₁₀ performed similar to the control (CK).(3)With the concentration of activated carbon increasing,the activity of GS increased and then decreased gradually in culture;The activities of GS were increased significantly (P<0.01) treated by 5.0 (B_5.0) and 7.5 (B_7.5) g/kg activated carbon;and protein content at B_7.5 was improved as well.(4)With activated carbon added increasing,the activities of SOD,POD and CAT also got a maximum and then changed very little with the culture time;The activities of SOD,POD and CAT at B_2.5 were much higher than that of CK.Therefore,we concluded that certain activated carbon could improve the metabolism,activities of protective enzymes and root growth.

---