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作 者 ：贾琳斐，杨 颖，李清宇，彭 晶，马婷婷，李 淼，段玉峰*

期 刊 ：西北植物学报 2012年 12期 页码：2486~2491

关键词：蒲公英；糖蛋白；自由基；抗氧化；

Keywords:Taraxacum mongolicum, glycoprotein, free radical, antioxidant,

摘 要 ：为了探讨蒲公英糖蛋白（TMGP）体内外抗氧化活性，采用H₂O₂、Fe²⁺、DPPH·和总抗氧化能力反应体系，检测蒲公英糖蛋白体外抗氧化活性，并与V_C进行比较；同时建立D-半乳糖所致衰老小鼠模型，测定并比较TMGP组与模型组的血清、肝脏和脑内MDA含量和SOD、CAT、GSH-Px活性。结果显示：（1）随着TMGP浓度增加，其Fe²⁺、H₂O₂和DPPH·清除率均有所增加，当TMGP浓度为1.72 mg/mL时，对H₂O₂、Fe²⁺清除率分别达到76.8%和55%，接近V_C水平；当TMGP浓度为1.5 mg/mL时，对DPPH·的清除率达到77%，也与V_C水平相当，说明TMGP具有一定的体外总体抗氧化能力。（2）将TMGP按低、中、高剂量分别给小白鼠灌胃，可显著增强小鼠体内血清、肝脏、脑组织中SOD、CAT、GSH-Px活性，降低MDA含量，从而提高衰老小白鼠体内的抗氧化能力。研究表明，TMGP具有明显的体内外抗氧化作用。

Abstract:We investigated the antioxidant effect of Taraxacum mongolicum glycoprotein (TMGP) in vitro and in vivo.The reaction system of H₂O₂,Fe²⁺,DPPH· and total antioxidant capacity was used to detect the antioxidant activity of TMGP in vitro and was compared with V_C.The contents of MDA and the activities of SOD,CAT,GSH-Px in plasma,liver and brain were respectively measured in aged mice model induced by D-galactose.The result showed that:(1)The scavenging capacity of TMGP for H₂O₂ and Fe²⁺ were enhanced as TMGP concentration increased.The H₂O₂,Fe²⁺ scavenging rates reached 76.8% and 55% respectively at 1.72 mg/mL TMGP,close to the level of V_C;When TMGP concentration at 1.5 mg/mL,the DPPH· scavenging rate reached 77% and it was near V_C;(2)The animal experiment showed that the content of MDA decreased and the SOD，CAT，GSH-Px activities increased in plasma,liver and brain in all TMGP groups,significantly increased the antioxidant capacity of mice.TMGP has obvious antioxidant effect in vitro and in vivo.

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