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作 者 ：方伏荣1,金 平1,王 峰2,聂石辉1,王 仙1,杜卫军3,张金汕1,吴志明4*

期 刊 ：西北植物学报 2013年 6期 页码：1079~1084

关键词：大麦；类钙调磷酸酶B互作蛋白激酶(CIPK)；克隆；非生物胁迫；实时定量PCR；

Keywords:barley, CIPK, clone, abiotic stress, real-time PCR,

摘 要 ：采用RT-PCR法从大麦抗旱品种‘甘啤7号’中克隆了1个类钙调磷酸酶B互作蛋白激酶（CIPK）基因HvCIPK1（GenBank登录号为JX679077）。序列分析表明,该基因全长1 359 bp,编码的蛋白含有452个氨基酸,分子量为51.05 kD,等电点为9.13。推断具有植物CIPK家族典型的功能结构域,在N端有一特异的催化结构域,该结构域在保守的氨基酸DFG和APE之间含有一个催化所需的活性环;同时C端区域存在一个独特的24个氨基酸组成的调节域,即NAF结构域。荧光定量分析结果表明,在PEG、NaCl和ABA处理下,HvCIPK1基因在大麦幼苗叶片中表达显著上调。推测HvCIPK1基因参与多种逆境信号的转导,可能在大麦的多种非生物胁迫响应中起着重要的作用。

Abstract:A protein kinase gene (calcineurin B-like proteins-interacting protein kinase,CIPK) was cloned from barely drought resistance variety ‘Ganpi 7’ by RT-PCR technology and named as HvCIPK1(GenBank accession JX679077).The length of the HvCIPK1 cDNA sequence is 1359 bp,and the predicted protein has 452 amino acids with an estimated molecular mass of 51.05 kD and an isoelectric point of 9.13.The protein contain an N-terminal kinase catalytic domain with a putative activation loop located between the conserved DFG and APE sequences,and the conserved 24-amino acid motif within the C-terminal non-kinase region of the CIPKs.Analysis of the gene expression by quantitative real-time RT-PCR (qRT-PCR) showed that the transcripts of HvCIPK1 were up-regulated in young barley leaves after stress treatments of polyethylene glycol,high NaCl and abscisic acid.The study suggested that the HvCIPK1 was involved in multiple stress response pathways in plants,revealing its key role in abiotic stress responses.

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