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作 者 ：高瑞芳, 程颖慧, 王 颖, 汪 莹, 国立耘, 章桂明

期 刊 ：植物病理学报 2016年 46卷 2期 页码：213-222

关键词：检疫性疫霉；DNA条形码；遗传距离；条码间隔；

Keywords:quarantine Phytophthora, DNA barcoding, genetic distance, barcoding gap,

摘 要 ：疫霉是世界关注的一类植物病原真菌。以疫霉属80个种122个菌株为研究材料,以ITS、CO1、EF-1α和β-tubulin 4个基因片段为候选DNA条形码,分析表明ITS、CO1基因的PCR扩增和测序成功率最高,分别为100%和96.7%;ITS、CO1和β-tubulin存在明显的条码间隔,但种间、种内距离频率分布存在较小重叠;种间、种内遗传距离Wilcoxon秩和检验结果认为各基因对种内遗传距离的效力相等,对种间遗传距离的区分能力为ITS>CO1>β-tubulin。CO1基因和ITS片段可同时作为11种检疫性疫霉的首选DNA条形码,β-tubulin基因可作为辅助DNA条形码。

Abstract:Phytophthora is the highest concern of plant pathogenic fungi. Four candidate genes of ITS, CO1, EF-1α and β-tubulin were tested with 122 strains from 80 species of Phytophthora genus to investigate the feasibility of serving as a DNA barcode. The results showed that the highest successful rate of PCR amplification and sequencing were from ITS and CO1 with 100% and 96.7%. Even the barcoding gaps of ITS, CO1 and β-tubulin were obvious. A smaller overlap between the frequency distribution of intra- and interspecific existed. The Wil-coxon rank test of intraspecific showed the equal distinguish effect, while the ability to distinguish interspecies was ITS>CO1>β-tubulin. ITS fragment and CO1 gene should be combined as barcodes for the eleven quarantine Phytophthora species, with assist of β-tubulin gene.

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