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作 者 ：王爱华，文晓鹏*

期 刊 ：西北植物学报 2012年 8期 页码：1698~1703

关键词：半夏；离体保存；缓慢生长；体细胞变异；

Keywords:Pinellia ternata, in vitro conservation, slow growth, somaclonal variation,

摘 要 ：以添加了不同浓度的甘露醇、PP₃₃₃和ABA的培养基对半夏试管苗进行缓慢生长法保存，并对保存材料再生后代的体细胞变异进行检测。结果显示，甘露醇、PP₃₃₃和ABA均能有效抑制试管苗生长，且存活率高；最佳浓度分别为甘露醇2%~4%，PP₃₃₃ 2.0 mg·L^-1，ABA 2.0~4.0 mg·L^-1。保存在添加了2%~4%甘露醇或2.0 mg·L^-1 PP₃₃₃培养基上的植株未检测到变异，而保存在添加了2.0~4.0 mg·L^-1 ABA培养基上的植株检测到1条新增标记和1条缺失标记，位点变异率为1.7%，个体变异率为30%。研究表明，ABA不宜用于半夏试管苗的缓慢生长法保存，但有助于新突变体的产生，在种质创新上具有特殊意义。

Abstract:Slow growth conservation of Pinellia ternata on medium by adding different concentrations of mannitol,PP₃₃₃,and ABA were carried out.Somatic variation of regenerated plantlets was also studied.The results show that:the three of them all could effectively retard the growth with a high surviving rate.The optimum concentration of mannitol,PP₃₃₃ and ABA were 2%~4%,2.0 mg·L^-1 and 2.0~4.0 mg·L^-1,respectively.No variations were found in the regenerated plantlets which were conserved on medium by adding mannitol 2% or PP₃₃₃ 2.0 mg·L^-1.While,one new marker and one absent marker were found on medium by adding ABA 2.0 mg·L^-1.The locus variation rate was 1.7% and the individual variation rate was 30%.Therefore,ABA was not suggested in slow growth conservation of Pinellia.However,it contributed to the generation of new mutant,and has a special significance in the innovation of P.ternate germplasm.

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