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作 者 ：闵长莉1,2，汪学军1,2，刘文博1

期 刊 ：西北植物学报 2012年 3期 页码：596~599

关键词：兰花；内生真菌；分离；抑菌活性；

Keywords:Cymbidium goeringii, endophytic fungi, isolation, antimicrobial activity,

摘 要 ：采用平板分离法从皖西大别山野生兰花的根、茎、叶中分离内生真菌32株，用琼脂块法对获取的菌株进行抑菌活性筛选，其中有4种兰花内生真菌对供试菌株有抑菌作用，占总分离菌株的12.50%，对抑菌活性较强的一株内生真菌进行形态学鉴定，结果表明它属于无孢类群。

Abstract:Thirty-two endophytic fungal strains were isolated from the roots,stems and leaves of wild Cymbidium goeringii by flat-panel separation.Agar block method was used to obtain the strain for antimicrobial activity screening.The results showed that four endophytic fungi (12.50% of the total strains isolated) had inhibitory activities to the test strains.We found a strong inhibitory activity of endophytic fungus.Morphological observation indicates that the strain belongs to Mycelia sterlia.

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