Abstract:Multiple alignment of 22 NAC transcription factors,including 14 abiotic stress related NAC transcription factors and 8 NAC transcription factors involved different biological functions ,was done and phylogenetic tree was built.Analysis and prediction of the amino acid composition,physical and chemical properties,hydrophilicity/hydrophobicity,conservative domain,the secondary structure,sub-cellular localization,and tertiary structure of 14 abiotic stress related NAC transcription factors were done with the help of bioinformatics tools.The results showed that 22 NAC transcription factors were divided into two groups，including a group of abiotic stress related NAC transcription factors and the rest ones;A,B,C,D and E subdomains constituted the NAC domain which were in N-terminus of 14 abiotic stress related NAC transcription factors,3 common motifs were also located in NAC domain;The C-terminus contained several conservative amino acid residues and many phosphorylation sites,was involved in transcriptional regulation;A,C,D subdomains were the main hydrophilic areas of abiotic stress related NAC proteins,and most of them were located in nucleus ,only a few ones in cytoplasm or mitochodria;α-helix and β-sheet were the primary secondary structure of abiotic stress related NAC proteins;Similarity of tertiary structure of RD26 and ANAC indicated that they would have the similar functions.