Abstract:A cadmium stress induced full-length cDNA Library from the Sedum alfredii was constructed by using improved SMART cDNA library construction protocol. Total RNA were extracted from S. alfredii under cadmium stress. After synthesizing the first-strand cDNA using limited amount of total RNA, the double-strand cDNA was amplified with long-distance PCR method. The full-length cDNA fragment was linked to versatile vector pYES2.0G, then the linked product was transferred into yeast INVSc1. The clones were randomly selected to sequence and bioinformatics analysis was carried out. The inserted fragments in the library are about 1000 bp length. This full-length cDNA Library provides a important tool for the study of the salt tolerance mechanisms of S. alfredii.