Abstract:78 fungal isolates, identified as Trichoderma spp., Rhizomucor spp., Aspergillus spp., Fusarium spp., Alternaria spp., Nigrospora oryzae and Botryosphaeria rhodina, respectively, were isolated from stained bamboo samples from North, South and Centre of China. The represent isolates were selected to conduct the biological characteristics study. The nutrition N and C had close relation to the hyphae growth and pigment secretion. Most of the fungi grew well at 28 ℃, and grew very slowly but not died at 5 ℃, at 40 ℃ grew slowly and nearly not survive. Isolate Y-50 survived and grew at 50 ℃, which showed that it had ability of surviving in extreme environment. The majority of fungi could grow at the condition of pH value 4 - 11 and the proper pH value was 5 - 8. The light factor had no significant impact on the growth of the fungi.