以湿地松器官性愈伤组织为材料，开展不定芽分化、生根与组织解剖学的研究。试验结果表明： 不定芽分化最佳培养基为TX+BA 0.5 mg·L-1+IBA 0.5 mg·L-1+30 g·L-1蔗糖； 不定芽同步发育最佳培养基为TX+IBA 0.5 mg·L-1+30 g·L-1蔗糖； 生根培养，初期以无大量元素培养基诱导生根、25 天后添加大量元素的培养程序可有效缩短生根培养时间，提高生根率； 亮白色、半紧实结构的外部形态及“拟分生组织"的内部结构是器官性愈伤组织的重要标志。
The organogenic calli of Slash Pine(Pinus elliottii) were used to study the differentiation, rooting and histological anatomy of adventitious buds.The result indicated that the best differentiation medium was TX+BA 0.5 mg·L-1+IBA 0.5 mg·L-1+30 g·L-1 sucrose， and the best synchronous development medium was TX+IBA 0.5 mg·L-1+30 g·L-1 sucrose. The medium without major elements was benefit to rooting of adventitious buds in the beginning of root initiation, and the rooting cycle had been shorten and rooting rate had been improved when major elements were supplemented 25 days after root initiation. The organogenic calli had signs of white and semi-solid appearance outside and large number of meristem inside.
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