Abstract:Leaves with systemic symptoms of ring or irregular spots and deformation (P-Ⅰ), and rolling leaves with yellowing inter-vein (P-Ⅱ), were collected from Hainan province and Beijing. The infected young leaves were triturated in a mortar with a pestle in 0.01M sodium phosphate buffer(pH7.2) containing antioxidate of 0.1% diethyldithioca-rbamate. The sap can infect both Nicotiana tobacum and N. tobacum cv. Xanthi-nc. And other herbaceous plants could also be infected with the two bridge hosts by mechnical transmission.Based on the morphology of the particles, serological reactions, physical properties and symptoms on differential hosts, P-Ⅰ and P-Ⅱ were considered to be CMV of Cucumoviruses and TMV of Tobamoviruses, signed by the symbol CMV-Pa and TMV-Pa, respectively.