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作 者 ：郑郁善

期 刊 ：林业科学 1998年 34卷 4期 页码：1-7

关键词：板栗；锥栗；顽拗性种子；种子萌发；ABA；贮藏蛋白质合成；

Keywords:C.mollissima, C.henryi,  Recalcitrant seed, Development, ABA, Storage protein,

摘 要 ：板栗和锥栗种子发育过程中内源ABA含量逐渐上升,达到高峰时间是板栗开花后105天、锥栗开花后100天,随后ABA含量均逐渐下降。果实成熟采收时ABA含量比高峰时小10倍。随着种子发育,种子和胚对外加ABA的敏感性也逐渐下降,10^-5mol/LABA可以完全抑制开花后100天的两个树种种子的发芽,但对成熟种子10^-2mol/LABA亦不能抑制发芽。ABA对成熟前期胚的贮藏蛋白质合成无影响,但能促进成熟中后期胚的贮藏蛋白质的积累作用,ABA维持板栗和锥栗贮藏蛋白质合成和积累作用表现在转录水平上。

Abstract:ABA contents increased obviously during the early developmental stages of C.mollissima and C.henryi seeds. The ABA contents of the seeds reached the peak of 1935ng/g at 105 days (C.mollissima) and 1573ng/g at 100 days (C.henryi) after flowering, then decreased gradually in both species. Moreover, the sensitivity to ABA of seeds and their isolated embryos decreased during development. The germination of two kinds of seeds 100 days after flowering inhibited by 15^-5mol/L ABA, and failed to completely inhibit even by 10^-2 mol/L ABA if the seeds harvested at full maturity. The effects of ABA in promoting the synthesis and accumulation of protein storage in embryos was at the transcriptional level, in the later process of mature.

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