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作 者 ：韩素芬

期 刊 ：林业科学 1996年 32卷 5期 页码：434-440

关键词：豆科树种；根瘤菌；结瘤；共生固氮；

Keywords:Tree legumes, Root nodule bacteria, Nodulation, Symbiotic nitrogen fixation,

摘 要 ：调查观察了48属122种豆科树种的结瘤情况,其中82种结瘤。采用VincentJM分离方法[1],从根瘤中分离获得了123个根瘤菌株的纯培养,各菌株的菌落特性和生长速度差异明显.经回接和交叉接种试验进一步确定它们为豆科树种根瘤菌。

Abstract:48 genera 122 speciss of tree legumes were examined for nodulation in field and seedling nursery. Among them, 82 species were found to be nodulated. 123 strains of root nodule bacteria from nodules of the trees were isolated following the method suggested by Vincent (1970). Isolates of rhizobia were purified and maintained on YEMA slants. Strains of rhizobia obviously differ from one another in characteristics of colonies and growth rate.

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