Abstract:Advances in biotechnology of Dendrobium orchid including tissue culture, flowering and germplasm preservation in vitro, protoplast culture and fusion, as well as genetic engineering were reviewed, based on the literature cited during two decade years. Meanwhile, subjects on the technology of plantlet transplantation, mycorrhizal technology, and secondarymetabolites in vitro are discussed in this paper to desert further research in order to provide well background for Dendrobium orchid industrialization. Furthermore, the swift development of biotechnology benefits to prompt the Dendrobium orchid breeding for new varieties.