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Effect of Brassinolide (BR) on the Antioxidant System of Amorpha fruticosa Seedlings under Petroleum Pollution

油菜素内酯对石油污染下紫穗槐苗木抗氧化系统的影响



全 文 :!23" !3#
 Vol.23  No.3
! " # $
ACTA AGRESTIA SINICA
   2015$  5%
  May.  2015
犱狅犻:10.11733/j.issn.10070435.2015.03.032
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67

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(BR)<ԃEðXBd(犃犿狅狉狆犺犪犳狉狌狋犻犮狅狊犪)…l^p\ùúIÕÖ,5WÞå©Zí
î

¤‹Œ‡eƒe¥åæ
(BR)<…l؈j2ŽÔ±¢,ˆ|^p\£!ñj^p\)¯"。€a+›:BR
ôœ‹¦Ñˆp\&D\£
(SOD)、^ {Y½;p\&£(APX)、;p\þ£(CAT)jYÙÆÚiû£(GR)I!
ñ

œ‹ùú^{Y½
(ASA)jiûbYÙÆÚ(GSH)¯ ",ù/XBd^ԃEðñ,æçP0.3mg·L-1Iƒ
e¥åæ‡e>azc

89:

XBd

ƒe¥åæ

YBԃEð
;^
p\ùú
;<,-=
:S793.2    >?@AB:A     >CD=:10070435(2015)03065704
犈犳犳犲犮狋狅犳犅狉犪狊狊犻狀狅犾犻犱犲(犅犚)狅狀狋犺犲犃狀狋犻狅狓犻犱犪狀狋犛狔狊狋犲犿狅犳犃犿狅狉狆犺犪犳狉狌狋犻犮狅狊犪
犛犲犲犱犾犻狀犵狊狌狀犱犲狉犘犲狋狉狅犾犲狌犿犘狅犾狌狋犻狅狀
HANYuanyuan1,HANGang2,LIKairong3
(1.InstituteofSoilandWaterConservation,NorthwestA&FUniversity,Yangling,ShaanxiProvince712100,China;
2.ColegeofForestry,NorthwestA&FUniversity,Yangling,ShaanxiProvince712100,China;
3.ColegeofResourceandEnvironment,NorthwestA&FUniversity,Yangling,ShaanxiProvince712100)
犃犫狊狋狉犪犮狋:Toexploretheeffectofbrassinolide(BR)ontheantioxidantsystemof犃犿狅狉狆犺犪犳狉狌狋犻犮狅狊犪seed
lingsunderpetroleumpolution,theindoorpottedexperimentwasusedbydippingrootsandspraying
leavesofseedlingswithdifferentconcentrationsofbrassinolide.Theresultsshowedthatbrassinolidesig
nificantlyincreasedtheantioxidantprotectionenzymeactivitiesofSOD,APX,CATandGR,thecontents
ofASAandGSHthatkepttheactiveoxygencontentofseedlingsinbalancetoavoidoxidativedamageto
cels.Themostsignificantconcentrationofbrassinolidewas0.3mg·L-1for犃犿狅狉狆犺犪犳狉狌狋犻犮狅狊犪.
犓犲狔狑狅狉犱狊:犃犿狅狉狆犺犪犳狉狌狋犻犮狅狊犪;Brassinolide;Soilpetroleumpolution;Antioxidantsystem
EFGH
:20140804;IJGH:20141223
KLMN

W÷›JñW÷-Ìß¿½¾

ƒ(2011040024);0„®W-„à.2?-Ì÷RKß
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(QN2011162)ÀÁ
OPQR

µ––
(1989),á,ÿ0Ò¸§,úËñ,mVÏÐW÷ñòRSÌÍ,Email:767253448@163.com;:6x× Authorfor
correspondence,Email:lkrzhxy@nwsuaf.edu.cn
  ÿ„x_͸~VIԃ۰4,f‡5íî’
“IŒŽ

#Íñ‰Ž~I(Ö¾

ÝÞ1Ô

Ô
ƒÀfjƒE;" Ú«!×

e4IYB5\ñ
ÀýØz{

ž&à"„Ð

Õ։e4ñòùúI
u)
[1]。
¥á<ԃEðI£¡8

ž&£¡Ë5
n?ƒ

0ÓEð&"à

én_§¿úûôOýI
Ö³

ƒe¥åæ
(brassinolide,BR)ôù/ž&I
^añ
[2]、
‡5ñ5ñ\z{I¦Ñ

¥á¸åC
<BRI^añÌÍmVÊ8fž&^、^ ÒPQ
^=V֎
[3],
æ<Þ^ԃEð>­áÄâÌ
Í

XBd
(犃犿狅狉狆犺犪犳狉狌狋犻犮狅狊犪)˜ÿ„zšª¾W
\`g¥

ðâÚ+ñ

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æ<ÿ„žŸ ¡â~V ¨

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üN^p\ùúvPiݞ&fa(8ýØIE
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ˆmV^p\£!ñj^p\)¯"

JKYBԃ
EðBREð14¾WjEðYB¦R5’LH

1 STUVW
1.1 Ö×STUkl
íîÞ2013$fÿ0~30„®W-„à.
…ž245W

_é{RíYB‹ý|ýEð&Õ
Ö

“F_ÿ„!2A\rǧ_!ׇIÀ]4
0~20cmÿEðYB,ˆHDEKqIe4“LÔ
ƒEð‡eÉ_10~20g·kg-1,‹¼3A‹ŒÔ
ƒË5Éu
:10,15,20g·kg-1(YB¹~)。ԃ
Eð&“_\rÇV04ƒ1Û°Iûƒ

fYB
¸¹¢

‘Û©¬10.54kg(¹~),’‹¼Iԃ
EðÉuJ**úԃEð&

§R§

n¤1$ñXBd…:_ÿ„!2A…ž。‹
¼4ABR‡e:0,0.1,0.3,0.5mg·L-1,‘A‡
e5Û,‘Û36,íˆùHØj2ŽÔ±Ë5[4]。
g¢W…lòÞv%×µ*Ù9å]É

1.2 m®e@UVW
°Ê…l8Uóô2.

9~P¤nŒ+ˆ

é
îÞ-70℃ˆƒyêRÔ¤。
ˆp\&D\£
(superoxidedismutase,SOD)
!ñˆJàBeyer[4]Ö³;;p\þ£(cata
lase,CAT)!ñJàTanidaÖ³[5];^ {Y½;p
\&£
(ascorbateperoxidase,APX)!ñJàSa
ruyama[5]Ö³;YÙÆÚiû£(glutathionere
ductase,GR)!ñJà Ding[5]Ö³;^ {Y½
(ascorbicacid,ASA)¯ "ˆJàTanaka[5]Ö
³

iûbYÙÆÚ
(reducedglutathione,GSH)¯
"JàElmanÖ³[5]。‘Az{ˆ~¡5~。
1.3 ijkl
°¤SPSS17.0ÉOonewayANOVA H5WÖãJK
,Duncan³5Wœ‹ñJK。¤
Origin9.0ø‰ÉO5Wø‰。
2 mnU,c
2.1 犅犚ÁöY€l¾)ÅèÀ0oÔÕ
2.1.1 BRÊSODšM@ÑÒ ݉1vÀ,‹Œ
ԃ‡eÉu
,3` BR ‡eË5¢,ü‰15
g·kg-1Ž0.1mg·L-1BR Ë5¢XBd…I
SOD!ñf8%2²€ÌŽ¶<à)u,|ýù
œ‹àÞ<à
(犘<0.05),¬t<àùú‰7.13%
~66.93%。ž&SOD!ñœ‹†÷,í\O2-’ñD\.­,
}؆}O2-<ž&IÔDIx¤。
<1 &è~{¬á€犅犚u)Á犛犗犇rÐoÔÕ
Fig.1 EffectsofdifferentBRconcentrationsonSOD
activityunderdifferentconcentrationsofpetroleumpolution
(A:10g·kg-1;B:15g·kg-1;C:20g·kg-1)
•

‹Œ4…wx+Ō#‹ŒË5hãRœ‹
(犘<0.05).Œ
Note:Differentlettersmeansignificantdifference
at0.05levelatthesametime.Thesameasbelow
2.1.2 BRÊCATšM@ÑÒ ݉2vÀ,‹
ŒÔƒEðÉu
,3`BR‡eË5¢,ü‰20
g·kg-1Ž0.1mg·L-1BR‡eË5XBd…I
CAT!ñf7%2²°FÝ̎¶<à)u,|ý
ùœ‹àÞ<à
(犘<0.05),¬t<àùú‰
7.58%~67.95%。!›fԃEðÉu_ 10
g·kg-1j15g·kg-1Ž,3`BR‡eùôœ‹¦
ÑXBd CAT !ñ,-f~eԃEðÉu20
g·kg-1Ž,0.1mg·L-1BR‡efXBdñ·á
#D’CAT!ñô.SÆ,"#ԃ4#ŽhI


|D’ô.ù/

2.1.3 BRÊ APXšM@ÑÒ ߉3nÅ,"
#ԃ‡eIùà

XBd…lI APX!ñœ…
†4

ԃEðÉu_10g·kg-1j15g·kg-1
Ž
,3`BR‡eË5¢XBd…lIAPX!ñœ
‹àÞ<à
(犘<0.05)。EðÉu}Ø20g·kg-1
Ž

çâ0.1mg·L-1BR‡eË5¢APX!ñf
ïAñ·#Ü朋àÞ<à
(犘<0.05)。!›f
ԃEðÉu_10g·kg-1j15g·kg-1Ž,3`
BR‡eùôœ‹¦ÑXBd…lIAPX!ñ,-
856
!3# µ––:ƒe¥åæ<ԃEðXBd…l^p\ùúIÕÖ
0.3mg·L-1BR‡eË5¢zœ‹,f~eԃE
ð20g·kg-1Ž,0.1mg·L-1¦ÑXBd…lI
APX!ñzœ‹。
<2 &è~{¬á€犅犚u)Á犆犃犜rÐoÔÕ
Fig.2 EffectsofdifferentBRconcentrationsonCAT
activityunderpetroleumpolution
<3 &è~{¬á€犅犚u)Á犃犘犡rÐoÔÕ
Fig.3 EffectsofdifferentBRconcentrationsonAPX
activityunderpetroleumpolution
2.1.4 BRÊGRšM@ÑÒ ݉4vÀ,"#
ԃ‡eIùà

XBd…lIGR!ñœ…†4。
‹ŒÔƒEðÉu
,3`BR‡eË5¢XBd
…IGR!ñœ‹àÞ<à(犘<0.05),¬t<à
ùú‰58.63%~255.14%。>D’GR!ñ,}Øçü H2O2x¤。
2.2 犅犚ÁöY€‚ÀbÅèÀ0oÔÕ
2.2.1 BRÊ ASAàs@ÑÒ ݉5vÀ,f
ԃEðÉu_10g·kg-1Ž,3`BR‡eË5
¢

ü‰0.1mg·L-1j0.5mg·L-1BRË5¢X
Bd…IASA¯"f7%2²€ÌŽ¶<à)
u

|ýùœ‹àÞ<à
(犘<0.05),0.3mg·L-1
BR‡eË5¢z›œ,J*SŒ#<àùú‰
85.95%,95.14%,80.17%j87.15%。fEðÉ
u}Ø15g·kg-1Ž,3`BR‡eË5¢,ü‰
0.1mg·L-1BRË5¢XBd…IASA¯"f7
%2²€ÌŽ¶<à)u,|ýùœ‹àÞ<à
(犘<0.05)。ԃEðÉuùàC20g·kg-1Ž,3
`BR‡eË5¢,ü‰0.5mg·L-1BRË5¢X
Bd…IASA¯"f10%2²€ÌŽ¶<à)
u

|ýùœ‹àÞ<à
(犘<0.05)。BR‡eôœ‹D!ԃEð4#XBd…lI
ASA¯"ùú。
<4 &è~{¬á€犅犚u)Á犌犚rÐoÔÕ
Fig.4 EffectsofdifferentBRconcentrationson
GRactivityunderpetroleumpolution
2.2.2 BRÊGSH às@ÑÒ ߉6nÅ,"
#ԃ‡eIùà

XBd…lI GSH ¯"œ…
†4

Eð|Ž~
,GSH ¯"|4。‹ŒÔƒEð
Éu
,3`BR‡eË5¢XBd…IGSH¯"
œ‹àÞ<à
(犘<0.05),¬t<àùú‰8.35%
~100.19%。D’XBd…
lIGSH¯"ùú,}Øçü!ñpIx¤。
3 œ
ԃEð4#ԟŠYB¯Éæ
、pHð、û>
Å

ÿjû>¿¯"œ‹†4
[1],
ƒYBk.

<ž&Iñ·jñ5Hñ¾nÕÖ

a(8ž&Ô
Íñ;øI!ñp

£2åI!ñp¯"ë)

<+
956
! " # $ !23"
<5 &è~{¬á€犅犚u)Á犃犛犃*åoÔÕ
Fig.5 EffectsofdifferentBRconcentrationson
ASAcontentunderpetroleumpolution
<6 &è~{¬á€犅犚u)Á犌犛犎*åoÔÕ
Fig.6 EffectsofdifferentBRconcentrationsonGSH
contentunderpetroleumpolution
Ív¾nÔD

·ÜÝ
[6]、
§[
[7]
<ԃEð…
lIÌÍzq
,5~20g·kg-1ԃ‡eË5Ô£…
lISOD,CAT,APXj GR!ñS<àœ‹
ƒ

f?ÌÍ8

díBRË5XBd…ISOD,
CAT,GRj APX!ñPQ ASA,GSH ¯"¶Ô
ƒEð‡en‹¬··ù

!›ÔƒEð4#Ž~
XO‰ž&^p\£I!ñj^p\)IÍñ

<
^p\ùú¾n-Ô

‹£Þž&lšIñ·jñ
5!×

ԃEð‡e|à

<^p\ùúI-Ô

,20g·kg-1IÕÖzà。
BRô¦Ña(8ž&I^p\éêx¤。Ï
›÷
[8]
ÌÍ+›
,BRË5Ic%fÕ4#v
é)SÑISOD,POD,CAT!ñ,Ïæ¦Ñž6


g«š
[9]
:;ÌÍKq

2ŽÔ±ƒe¥å
æf¥ˆSe†¦Ñ‰a(8OP„…SOD,POD
jCATI!ñ。Ì«¦[10]ÌÍ+›,BRË5v
P:;¦Ñc%„…ˆù8 ASA¶ GSH ¯"æ
¦Ñc%„…<ƒp4#I3ñ

f?ÌÍ8
,3
`ԃ‡eÉu

¬t<à

XBd…lí0.1~
0.5mg·L-1BR‡eË5¢,SOD,CAT,APXj
GR!ñS<à✋¦Ñ,ASA,GSH ¯"#â
œ‹ùú

â>4çüž&2å;ø!ñp

()+
Ívlšóô

Pç0.3mg·L-1IBR‡eË5
^p\éê>azc

XBd…l^p\£!ñj
^p\)¯"I¦Ñù/‰|fYBԃEð4#
<ž&+Íéêx¤

()‰XBd2å!ñp
u)

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}
Ø^ԃEðIx¤

4 m
  ԃEð4#,BRôœ‹¦Ñ^p\éê
£SOD,APX,CATj GRI!ñ,ùú^p\)
ASA,GSH ¯"。æç0.3mg·L-1IBRË5<
ԃEð4#XBdI^p\ùúéêz2

žŸ>?
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\éêÖ­
[J].]÷-.,2014,31(4):632640
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IÖ­
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[J].8¸®÷-.,2006,39(9):18721877
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[10]Ì«¦,.ØÌ,3™,.24+ƒe¥åæ<ƒp4#c
%„…ˆù^p\ùúIÕÖ
[J].8¸®÷-.,2008,41
(1):153161

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