---

作 者 ：王歧, 段碧华, 杨瑞, 赵耀荣, 张露

期 刊 ：草地学报 2013年 21卷 5期 页码：998-1004

关键词：几丁质酶；放线菌；草坪草；根腐病；筛选；鉴定；抑制作用；

Keywords:Chitinase, Actinomycetes, Turfgrass, Root rot disease, Screening, Identification, Inhibition,

摘 要 ：通过对草坪土壤中产几丁质酶放线菌的筛选、鉴定及对4种草坪草根腐病菌的抑制作用研究,为草坪草根腐病的生物防治提供指导。通过菌落法和菌饼法筛选得到1株产几丁质酶活性较强的放线菌菌株Bn035,其降解圈相对直径分别为3.60和3.42;采用DNS法测量几丁质酶活性发现,第6 d时酶活达到最大为1.23 U·mL^-1;菌体和粗酶液均对草坪草根腐病菌产生较强的抑制作用;根据形态学特征、培养特征、生理生化特征和16S rDNA序列比对综合分析,菌株Bn035为皱孢链霉菌(Streptomyces scabrisporus)。菌株Bn035产几丁质酶活性较强且抑菌效果较好,表明其在真菌性根腐病生物防治方面具有潜力。

Abstract:Chitinolytic actinomycetes from lawn soil were screened and identified and their inhibitory activities on four types of turfgrass root rot disease were studied. Results showed that screened Bn035 strain had stronger chitinase activity than the other four strains; the relative diameter of chitin-lytic zone was 3.60 and 3.42, respectively. Its chitinase activity in culture broth reached a maximum value of 1.23 U·mL^-1 on the 6^th day. Crude enzyme solution and thallus of Bn035 strain showed stronger antifungal activity against all tested fungi. The Bn035 strain was identified as Streptomyces scabrisporus by morphological, cultural, physiological, biochemical and phylogenetic characteristics. The Bn035 strain had strong chitinase activity and inhibitory activity that suggested its potential application in the biological control of turfgrass fungal root rot disease.

---