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作 者 ：李从强;林刚;李克秀;宋运淳;熊志勇;何光源

期 刊 ：武汉植物学研究 2006年 24卷 1期 页码：1-5

关键词：玉米；二倍体多年生类玉米；异染色质钮；荧光原位杂交；重复序列；

Keywords:Zea mays, Zea diploperennis, Repetitive DNA sequence, Fluorescence in situ hybridization, Heterochromatic knob,

摘 要 ：利用组成玉米异染色质钮的180-bp重复序列和TR-1元件以及45SrDNA对玉米自交系F107、GB57、二倍体多年生类玉米及其远缘杂交后代的染色体进行荧光原位杂交,确定了3种重复序列在亲本染色体上的分布;同时对远缘杂交后代进行了细胞学鉴定,通过荧光信号在染色体上的位置,证实远缘杂交后代中异源种质的染色体来源;讨论了异染色质钮重复序列对玉米和其野生种杂交后代外源染色体整合和染色体行为等方面研究的应用。

Abstract:Fluorescence in situ hybridization(FISH) was carried out on mitotic chromosomes of Zea diploperennis(DP),maize inbred lines F107,GB57 and their hybrids using knob-associated tandem repeats,180-bp repeats and TR-1 elements,together with 45S rDNA as probes.The distribution patterns of the three repeated sequences were analyzed on parental chromosomes.The hybrids of maize and DP were(identified by) FISH.According to the hybridization signals on chromosome positions,the origin of foreign idioplasm was confirmed.The application using knob-associated tandem repeats as excellent chromosomal markers on detecting the integration of foreign chromosome and the chromosomal activity at meiosis was also discussed.

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