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作 者 ：李兆波;;李伟;顾舒平;操瑜;;尹黎燕;

期 刊 ：植物科学学报 2011年 29卷 2期 页码：226-233

关键词：低浓度CO2；水车前；黑藻；光合特性；光合酶；光合速率；氧抑制；叶绿素荧光；

Keywords:LowCO2, Otteliaalismoides, Hydrillaverticillata, Photosyntheticcharacteristics, Photosyntheticenzymes, Photosynthesisrate, O2inhibition, Chlorophyllfluorescenc,

摘 要 ：本实验通过对沉水植物水车前(Ottelia alismoides)和黑藻(Hydrilla verticillata)进行低浓度CO₂诱导,研究其光合特性的变化。研究显示,诱导后,水车前的PEPC/Rubisco活性比值由0.45上升到4.17,黑藻由0.47上升到4.17,两种植物的C₄途径光合酶PEPC和NAD-ME的活性升高了10倍左右,其他C₄光合酶如NAD-MDH和PPDK的活性也大幅升高;诱导后,水车前和黑藻的其他光合特性也发生了显著变化:净光合速率比对照分别提高了50.8%和74.1%,O₂对处理组光合速率的抑制分别为对照组的35%和60%,叶绿素荧光基本参数也有显著性变化。研究结果表明,经低浓度CO₂诱导,水车前和黑藻光合碳同化途径可能由C₃转变成为C₄。

Abstract:Photosynthetic characteristics of Ottelia alismoides and Hydrilla verticillata were studied after the two species were incubated under low CO₂ conditions.The PEPC/Rubisco ratio of O.alismoides increased from 0.45 to 4.17 and of H.verticillata increased from 0.47 to 4.17 after low CO₂ induction.The activity of PEPC and NAD-ME increased more than 10-fold and other C₄ enzymes such as NAD-MDH and PPDK also increased significantly after low CO₂ induction.Other photosynthetic characteristics also changed significantly.The net photosynthetic rate of O.alismoides and H.verticillata increased by 50.8% and 74.1%,respectively,and the oxygen inhibition on photosynthetic rate of the two species decreased to 35% and 60%,respectively,after low CO₂ induction.The chlorophyll fluorescence parameters also changed after low CO₂ induction.All these facts indicated that photosynthetic carbon assimilation of O.alismoides and H.verticillata might shift from C₃ to C₄ after low CO₂ induction.

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