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作 者 ：焦健;高庆荣;张爱民;郝媛媛;王大伟;王霖;邱新民

期 刊 ：作物学报 2007年 33卷 08期 页码：1267-1271

关键词：细胞质效应；雄性不育系；光合作用；日变化；

Keywords:Cytoplasmic effect, Male sterile lines, Photosynthesis, Diurnal changes,

摘 要 ：利用2套同核异质的K、T、V、CHA型不育系及其保持系，对其不同生育时期净光合速率（P_n）、气孔导度（G_s）、蒸腾速率（Tr）、水分利用率（WUE）、光量子通量密度（PFD）和细胞间隙CO₂浓度（C_i）、气孔限制值（L_s）等光合、生理参数的日变化及其差异进行比较研究，探讨不同小麦雄性不育类型的胞质效应。结果表明，供试材料净光合速率的日变化均呈双峰曲线，有明显的光合“午休”现象，P_n与G_s、C_i的相关系数分别为0.8187、0.8136，呈显著正相关（r_0.05=0.8110），与L_s的相关系数为－0.8496，呈显著负相关（r_0.05=0.8110），P_n的午间下降伴随着G_s、Ci的下降和L_s的升高，表明其主要受气孔因素限制；与保持系的Pn值相比，CHA型和K、T、V型不育系分别降低0.88、2.76、3.30和2.04 μmol CO₂ m^-2 s^-1，产生不同程度的负效应，尤以T型和K型最明显。在水分利用率上，K、T、V型不育系较保持系降低0.94~1.54 μmol CO₂ mmol^-1 H₂O，负效应显著；CHA型不育系较保持系低0.36 μmol CO₂ mmol^-1 H₂O，差异不显著，说明CHA对旗叶水分利用率无明显的不良影响。Pn值因母本基因型的不同而异，冀5418及相应不育系的Pn值较太911289的高，差异达显著或极显著水平。因此，选择优势效应大的保持系回交，有利于降低不育胞质对小麦光合功能的负效应。

Abstract:

In order to study the cytoplasmic effects of male sterile wheat, male sterile lines of CHA (chemical hybridizing agent) and cytoplasmic K (Aegilops kotschyi), T (Triticum timopheevi), V (Aegilops ventricosa) types of male sterile wheat at different developmental stages were compared with their maintainer lines in the diurnal changes of photosynthetic and physiological parameters, comprising net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r), water use efficiency (WUE), photon flux density (PFD), intercellular CO₂ concentration (C_i), atmospheric CO₂ concentration (C_a), leaf temperature (T_i), atmospheric temperature (T_a), and stomatal limiting value (L_s). And these parameters may reflect the photosynthetic efficiency of the leaves and environmental adapting capability of the plant. The experiment was conducted to analyze the diurnal changes and the physiological mechanism of photosynthetic midday-depression, the response to male sterile cytoplasms of female parent genotypes and the differences of photosynthetic and physiological parameters of 4 male sterile lines and their maintainer lines, which can reflect differences of heredity of 4 male sterile cytoplasms under the same nuclear background and also exhibit differences of the effect of nuclear-cytoplasmic interaction. The result indicated that, the curve of diurnal changes of P_n presented two peak values, with one peak at 10:00 higher than the other at 14:00. And the phenomenon of photosynthetic midday-depression of leaf happened at noon. The diurnal changes of Pn was positively correlated with G_s, C_i and negatively correlated with L_s, and the correlation coefficients were 0.8187*, 0.8136*, and －0.8496* respectively. Accompanied by the decline of G_s, C_i and the raise of L_s, P_n declined distinctly in midday, which means the limitation of Pn by stomatal factor. Compared with their maintainer lines, CHA and K, T, V-male sterile lines had lower P_n values, decreasingly 0.88, 2.76, 3.30, and 2.04 μmol CO₂ m^-2 s^-1 respectively, indicating that the CHA and K, T, V-cytoplasm has negative effects on Pn with different extent, especially T and K-types. K, T, V cytoplasmic male sterile lines had lower WUE values than their maintainer lines, decreasingly 0.94–1.54 μmol CO₂ mmol^-1 H₂O, and indicating that K, T, V-cytoplasm has significantly negative effects on WUE. Compared with the maintainer lines, CHA-sterile lines has no significant difference in WUE values, indicating chemical hybriding agent (BAU-9403) has no significantly negative effects on the water use efficiency of wheat flag leaf. The influences of damage caused by BAU-9403 were less than the negative effects of other male sterile cytoplasms. Moreover, the Pn value varied between genotypes of different female parents, Pn of Ji 5418 and its CMS lines showed significant or highly significant differences with that of Tai 911289, indicating that genotypes of female parents have different effects on different cytoplasms in Pn. This result also demonstrates the enormous potential for improving the photosynthetic capability in Ji 5418. Therefore, it is better to reduce the negative effects of male sterile cytoplasms on photosynthetic function of wheat by selecting preponderant recurrent parent to make backcross in a breeding project.

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