为了探明Bt棉晶体蛋白在细胞中定位的特异性，揭示Bt棉抗虫稳定性，以转Bt基因棉GK-12为试材，采用免疫细胞定位的方法，在亚细胞结构水平上研究Bt蛋白的定位取向。结果表明，Bt蛋白主要分布在细胞质和细胞壁间隙中，并且，在细胞质中的分布密度高于细胞壁间隙；在细胞质中，晶体蛋白主要分布在细胞膜、内质网、叶绿体和前质体上，并且在叶绿体和前质体上面高密度集中分布。说明转Bt基因棉GK-12叶肉细胞中，外源晶体毒蛋白的定位取向于惰性细胞器—叶绿体及其前体。
For the sake of proving the localization character of Bt crystal protein in transgenic Bt cotton cell and illustrating the insect resistance stability of Bt cotton, the study on the sub-cellular orientation of the Bt crystal protein in transgenic Bt cotton cell was conducted by means of immunocyte localization. The results showed that the Bt crystal protein were mostly located in cytoplasm and the intercellular space of Bt transgenic cotton GK-12 cotyledon mesophyll cells. The distribution density was larger in cytoplasm than in the intercellular space. In cytoplasm, the crystal protein was mainly distributed on the cell membrane, endoplasmic reticulum, chloroplast and plastid, especially on the surface of chloroplast and plastid with a high density. From above, we know that the Bt. crystal protein is straightly located onto inertial organelle—chloroplast and its plastid.
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