玉米耐盐品种登海9号和盐敏感品种浚单18在含0、50、100、150和200 mmol L-1 NaCl的营养液中萌发生长, 采用等离子质谱分别测定其萌动种子种皮、胚、胚乳和幼苗根、根颈、叶中Na+、K+、Ca2+的含量。结果表明, 随着培养液中NaCl浓度的增加, 玉米体内Na+含量逐渐升高, 在幼苗中表现地下部(根和根颈)显著高于地上部(叶); 在萌动种子中, 胚中Na+积累量显著高于种皮和胚乳。根系积累Na+能力较强, 胚拒Na+能力较弱, 种皮具有一定的Na+累积能力。随NaCl浓度的增加, K+和Ca2+含量逐渐降低, 尤其是Ca2+含量急剧减少, 达38.4%~55.9%(登海9号)和65.6%~78.2%(浚单18)。玉米根、根颈、种皮的Na+积累能力、叶的拒Na+能力和幼苗选择吸收Ca2+的能力可能与品种耐盐性有关。
Maize (Zea mays L.) seeds of a salt tolerant cultivar Denghai 9 and a salt sensitive cultivar Jundan 18 were germinated and grown in 1/4 and 1/2 Hoagland solutions with 0, 50, 100, 150, and 200 mmol L-1 NaCl, respectively. The contents of Na+, K+, and Ca2+ in testa, embryo, endosperm, roots, root crown, and leaf were measured by ICP Mass. The Na+ content increased accordantly with the NaCl concentration in culture solution. In young seedlings, Na+ content in root and root crown was higher than that in leaf; in germinating seeds, it was significantly higher in embryo than in testa and endosperm. The capacity of Na+ accumulation in roots was strong, while the capacity of Na+ rejection in embryo was weak. The testa could also accumulate some Na+. The K+ and Ca2+ contents in germinating seeds and young seedlings of maize decreased obviously under NaCl stress, and the Ca2+ content showed changes in a large scale, which was 38.4%–55.9% in Denghai 9 and 65.6%–78.2% in Jundan 18. The capacities of Na+ accumulation and rejection as well as the Ca2+ selective absorption might be related to salt-tolerance of maize cultivars
全 文 ： ACTA AGRONOMICA SINICA 2008, 34(2): 333−336 http://www.chinacrops.org/zwxb/
ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@chinajournal.net.cn
:
(973
) (2006CB101700);
(2004BA520A08);
(30471025); (NYHZX07-003);
(2006QAD02A09); !#$%&(2006790()
: )*(1963–), +, !,-. /, 012345/6/7 8Tel: 13505346116; 9::!;<,=> 2 (;<
?/5@8E-mail: sddzsxf@163.comA
* BC4D(Corresponding author): EFG(1953–), H, IJ, KL45/6/78Tel: 0538-8241591; E-mail: stdong@sdau. edu. cn
Received(MNOP): 2007-04-16; Accepted(QROPS: 2007-09-16.
DOI: 10.3724/SP.J.1006.2008.00333
Na+K+ Ca2+
1,2 1*
2
2
(1
, 271018; 2, 253023)
: 9
18 050100150 200 mmol L−1 NaCl
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Relationship between Changes of Na+, K+, and Ca2+ Contents during
Seed Germination and Salt Tolerance in Maize
SHANG Xue-Fang1,2, DONG Shu-Ting1,*, ZHENG Shi-Ying2, and WANG Li-Yan2
(1 Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, Shandong; 2 Dezhou College, Dezhou 253023, Shandong,
China)
Abstract: Maize (Zea mays L.) seeds of a salt tolerant cultivar Denghai 9 and a salt sensitive cultivar Jundan 18 were germinated
and grown in 1/4 and 1/2 Hoagland solutions with 0, 50, 100, 150, and 200 mmol L-1 NaCl, respectively. The contents of Na+, K+,
and Ca2+ in testa, embryo, endosperm, roots, root crown, and leaf were measured by ICP Mass. The Na+ content increased ac-
cordantly with the NaCl concentration in culture solution. In young seedlings, Na+ content in root and root crown was higher than
that in leaf; in germinating seeds, it was significantly higher in embryo than in testa and endosperm. The capacity of Na+ accumu-
lation in roots was strong, while the capacity of Na+ rejection in embryo was weak. The testa could also accumulate some Na+.
The K+ and Ca2+ contents in germinating seeds and young seedlings of maize decreased obviously under NaCl stress, and the Ca2+
content showed changes in a large scale, which was 38.4%–55.9% in Denghai 9 and 65.6%–78.2% in Jundan 18. The capacities of
Na+ accumulation and rejection as well as the Ca2+ selective absorption might be related to salt-tolerance of maize cultivars
Keywords: Maize; Salt stress; Na+ content; K+ content; Ca2+ content; Seed germination
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Fig. 1 Na+ content in geminating seed and young seedling of maize under different NaCl concentrations
2 :
Na+K+ Ca2+ 335
1 NaCl
K+
Table 1 K+ content in germinating seeds and young seedlings of maize under different NaCl concentrations (mg g−1)
NaCl NaCl concentration
Cultivar
Part of seed or seedling 0 50 mol L−1 100 mol L−1 150 mol L−1 200 mol L−1
Testa 4.41
0.60 2.58
0.99 2.18
0.78 2.03
0.42 1.20
0.95
Embryo 37.47
0.69 32.57
2.34 29.90
0.78 24.85
1.13 18.51
1.17
Root 44.27
3.66 34.24
2.80 30.59
3.58 25.91
2.00
−
Root crown 36.00
1.84 25.23
1.91 25.00
2.23 21.57
1.65
−
Leaf 40.00
1.20 39.53
1.56 25.71
1.55 23.68
1.65
−
9
Denghai 9
Mean 32.43 26.83 22.68 19.61
−
Testa 3.46
0.65 1.39
0.79 1.28
0.44 1.09
0.41 0.90
0.24
Embryo 37.23
1.54 31.43
1.37 22.06
1.29 20.00
1.35 16.91
0.96
Root 34.95
1.87 33.25
1.37 27.97
1.09 21.18
1.77
−
Root crown 35.96
1.55 28.00
1.19 22.67
2.34 19.39
1.25
−
Leaf 50.00
1.16 35.01
1.75 31.00
1.12 30.89
1.77
−
18
Jundan 18
Mean 32.32 25.83 21.00 18.51
−
2 NaCl
Ca2+
Table 2 Ca2+ content in germinating seeds and young seedlings of maize under different NaCl concentrations (mg g−1)
NaCl NaCl concentration
Cultivar
Part of seed or seedling 0 mol L−1 50 mol L−1 100 mol L−1 150 mol L−1 200 mol L−1
Testa 1.78
0.99 0.91
0.21 0.76
0.18 0.62
0.12 0.57
0.22
Embryo 1.11
0.40 0.84
0.18 0.72
0.16 0.59
0.18 0.57
0.12
Root 14.19
1.29 8.47
1.39 7.92
0.90 7.05
1.91
−
Root crown 4.00
1.38 3.59
1.67 2.99
0.79 2.97
1.06
−
Leaf 6.67
0.77 4.52
0.53 2.70
0.40 1.99
0.81
−
9
Denghai 9
Mean 5.89 3.63 3.05 2.60
−
Testa 1.38
0.60 0.64
0.21 0.58
0.18 0.53
0.29 0.43
0.20
Embryo 0.85
0.13 0.75
0.11 0.64
0.15 0.55
0.11 0.51
0.20
Root 18.57
1.30 12.12
1.23 11.41
1.70 5.25
0.53
−
Root crown 5.91
1.10 4.00
0.95 2.71
1.29 2.32
0.85
−
Leaf 6.88
0.88 2.37
1.43 1.87
0.96 1.16
0.39
−
18
Jundan 18
Mean 6.88 2.37 1.87 1.50
−
Ca2+ 32.3%~70.2% (
9 )
65.6%~83.1% ( 18),
40.3%~50.7%
(
9 )
34.1%~69.1% ( 18)
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336 34
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