采用PCR、RT-PCR以及其他分子生物学方法，以甘蓝基因组DNA和柱头cDNA为模板对MLPK基因进行扩增克隆，首次得到长度分别为1 615 bp和1 294 bp的基因片段。序列分析表明，甘蓝MLPK与芜菁MLPK的cDNA序列相似性达98%，前者的内含子数为4个，比后者少了3个；同时，在前者内含子中发现了不同于典型的GU-AG规则的新的序列，即第3、4个内含子5′端碱基是TA、CG，3′端碱基为CAGG、GT，推测甘蓝MLPK的mRNA具有独特的剪切机制。这为甘蓝自交不亲和分子机理研究提供了新内容。
The DNA and cDNA fragments of M locus encoding protein kinase were initially amplified from genomic DNA and stigma cDNA in Brassica oleracea by using PCR , RT-PCR and other molecular methods. Their lengths were 1 615 bp and 1 294 bp respectively. For the first time, sequence analysis indicated that there was 98% identity of MLPK cDNA between Brassica oleracea and Brassica rapa. In Brassica oleracea, the MLPK gene contained four introns, which were three introns less than those in Brassica rapa. Moreover, in two ends of introns of MLPK gene from Brassica oleracea, there were new base sequences which didn’t comply with typical GU-AG rule: TA and CG existed in the 5′ end of the third and the fourth intron of MLPK gene; CAGG and GT were found respectively in the 3’ end of the sequences. So we speculated that these new base sequences may have relation to a new splice process. These results provided some new insight into the molecular mechanism of self-incompatibility in Brassica oleracea.
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