全 文 ：V o l. 29, N o. 3
pp. 391—396　M ay, 2003
作　物　学　报
A CTA A GRONOM ICA S IN ICA
第 29 卷 第 3 期
2003 年 5 月　391—396 页
Genetic D iversity of El ite M a ize Germ plasm for Resistan t to SCM V
W AN G Zhen2H ua2 　L I X in2H ai1 　 YU AN L i2X ing1 　HAN X iao2Q ing3 　 ZHAN G Sh i2
H uang1, 3
(1 Institu te of C rop B reed ing and Cu ltiva tion, Ch inese A cad emy of A g ricu ltu ral S ciences, AM B ION E T 2Ch ina L ab. , K ey L aboratory of C rop Ge2
netics and B reed ing , M inistry of A g ricu ltu re, B eij ing 100081, Ch ina; 2 Colleg e of A g ronomy , N ortheast A g ricu ltu ral U niversity , H arbin, H ei2
longj iang 150030, Ch ina; 3 T ang shan Institu te of A g ricu ltu ral S ciences, T ang shan, H ebei 063001, Ch ina)
Abstract　Fo rty2six elite m aize (Z ea m ay s L. ) inbred lines w ere evaluated fo r react ion to sugarcane mo saic
virus under field condit ions w ith art ificial inocu lat ion in 1999 and 2000. T he resu lts show ed that 8 lines
(K22, CN 962, P138, Q i318, Zhongzi01, J inhuang96B, Q i319, and Pa405)w ere h igh ly resistan t to SCM V ,
and 7 ( H an21, Zhongzi03, H an23, N ongda178, H uobai, K12, and H uangzao4 ) w ere resistan t. T he
germp lasm basis fo r resistance to SCM V in 46 inbreds w as invest igated by SSR m arkers. T he average num 2
ber of alleles per SSR locus w as 3. 43 w ith a range from 2 to 10. T he U PGM A cluster analysis show ed that
the 46 inbreds cou ld be classified in to 7 dist inct group s w ith several subgroup s, w h ich w ere generally consis2
ten t to their know n pedigree info rm at ion and breeder’s experiences. F ifteen resistan t inbreds w ere found in 4
group s (A , B , E, G) , w h ich cou ld be used in germp lasm imp rovem ent fo r SCM V resistance based on hetero t2
ic pat tern, respect ively. Group E and subgroup G II w ere iden t ified as two impo rtan t sources of resistance,
w h ich can be emp loyed to syn thesize the compo sites fo r SCM V resistance. T he study p rovided usefu l info r2
m ation fo r germp lasm imp rovem ent fo r resistance to SCM V.
Key words　Z ea m ay s L. ; SSR m arker; H etero t ic group; Sugarcane mo saic virus; R esistance evaluat ion
玉米抗甘蔗花叶病毒资源的遗传多样性研究Ξ
王振华2　李新海1　袁力行1　韩晓清3　张世煌1, 3
(1中国农业科学院作物育种栽培研究所, AM B ION ET 中国实验室, 农业部作物遗传育种重点开放实验室, 北京　100081; 2东北农业大学农学
院; 黑龙江哈尔滨 150030; 3河北省唐山市农业科学研究所, 河北唐山 063001)
摘　要　利用人工接毒方法对 46 份我国主要玉米自交系进行了两年抗甘蔗花叶病毒鉴定, 筛选出高抗系 8 份 (K22、
CN 962、P138、齐 318、中自 01、金黄 96B、齐 319、Pa405) , 抗病系 7 份 (旱 21、中自 03、旱 23、农大 178、获白、K12、黄早
四)。用 SSR 标记研究了 46 份自交系的遗传多样性。49 对引物共检测出 168 个等位基因变异, 每对引物检测等位基因
2～ 10 个, 平均为 3143 个。U PGM A 聚类分析表明, 供试自交系可分为 7 群, 划群结果与系谱和育种家经验基本相符。15
份抗病毒自交系分散于 4 群 (A、B、E、G) , 依据杂种优势原理, 可用于改良同一群内感病系的抗性; 其中群 E 和亚群 G II
被鉴定为抗病毒种质, 可用于组建抗病群体。本文的研究结果为抗甘蔗花叶病毒玉米种质改良提供了重要信息。
关键词　玉米; SSR 标记; 杂种优势群; 甘蔗花叶病毒; 抗性鉴定
中图分类号: S513　　　文献标识码: A
　　M o saic d isease, cau sed by sugarcane m o saic
viru s (SCM V ) , is one of the m o st im po rtan t d is2 eases of m aize in Ch ina, w h ich cau ses 10%～ 15%yield lo sses annually. It w as repo rted as a severΞFoundation item: A sian M aize B io techno logy N ETwo rk (AM B ION ET ) P ro ject.
Biography: 王振华 (19652) , 男, 黑龙江海伦人, 副教授, 硕士生导师, 研究方向: 玉米遗传育种。3 Co rresponding autho r
Received (收稿日期) : 2002204226, A ccep ted (接受日期) : 2002204206.

disease of m aize in 1960s, and becam e m o re seriou s
in 1980s and 1990s. U p to date, the d isease has
been sp read in severa l p rovinces[ 1 ]. SCM V is na tu2
ra lly t ran sm it ted by aph ids in a non2persisten t
m anner. It becom es especia lly severe w hen m aize
is p lan ted in con t inuou s cropp ing system. A cco rd2
ing to the taxonom y of Shuk la et a l. ( 1989) [ 2 ] ,
m aize dw arf m o sa ic viru s (M DM V ) stra in B w as
reiden t if ied as SCM V. SCM V is m o re p reva len t
than M DM V in Ch ina and Eu rope, and M DM V is
p redom inan t ly d ist ribu ted in sou thern parts of
U SA [ 3, 4 ]. It is im po ssib le to con tro l SCM V direct ly
w ith chem ica ls. L ikew ise, the a t tem p t to con tro l
aph ids is no t effect ive becau se of the non2persisten t
m ode of viru s t ran sm ission. D evelopm en t of resis2
tan t hyb rids is the effect ive st ra tegy to con tro l
SCM V.
Studies on the resistance to M DM V and
SCM V have been conducted w ith U. S. and Eu ro2
pean m aize germ p lasm [ 4, 5 ]. Eva lua t ion fo r resis2
tance to SCM V in Ch inese m aize germ p lasm has
been done, bu t these eva lua t ion s w ere m ain ly con2
ducted w ith loca l variet ies and released hyb rids[ 6 ].
How ever, lit t le is know n on genet ic d iversity of
m aize germ p lasm fo r resistance to SCM V. T he in2
fo rm at ion can grea t ly facilita te germ p lasm im 2
p rovem en t and b reed ing act ivit ies fo r resistance to
SCM V.
Genet ic rela t ion sh ip can be est im ated by
analysis of ped igree, hetero sis, m o rpho log ica l
t ra its, isozym es o r u sing m o lecu lar m arkers. Since
1980s, the genet ic base of Ch inese m aize
germ p lasm has been invest iga ted by exam in ing per2
cen tage of paren ta l lines in their hyb rids u t ilized in
d ifferen t t im es, and hetero t ic group ing has been
clu stered by pedigree and geograph ic deriva2
t ion [ 7, 8 ]. DNA 2based m arkers p rovide a pow erfu l
too l in the assessm en t of the genet ic rela t ion sh ip s
am ong the b reed ing m ateria ls. Sim p le sequence re2
pea ts (SSR s) have p roven to be h igh ly po lym o rph ic
and u sefu l as genet ic m arkers in m any p lan t species
includ ing m aize [ 9～ 12 ]. SSR s rep resen t the app roach
fo r the iden t if ica t ion and pedigree va lida t ion of
m aize geno types com pared to o ther PCR 2based
m ethods. T he pat tern s of genet ic d ivergence by
SSR s w ere con sisten t w ith their know n pedi2
gree [ 12, 13 ]. T he ob ject ives of the study w ere (1) to
eva lua te 46 elite inb red lines fo r react ion to SCM V
under field condit ion s w ith art if icia l inocu la t ion;
(2) to ana lyze the genet ic d iversity by SSR s and to
assign them to hetero t ic group s, and (3) to p ro2
vide a stra tegy of germ p lasm im p rovem en t fo r re2
sistance to SCM V.
1　M a ter ia ls and M ethods
111　Plan t ma ter ia ls
Fo rty2six inb red lines, w h ich are cu rren t ly
u sed in Ch inese m aize b reed ing p rogram w ere in2
cluded in the study (T ab le 1). T he inb red lines
w ere m ain ta ined by self ing from seed of ind ividua l
ears.
Table 1　　Evaluation of 46 inbred l ines for resistance
to SCM V (1999 and 2000, Tangshan)
N o. L ine
1999 2000 A verage overtwo years
D I% RE D I% RE D I% RE
1 K22 0. 0 HR 0. 0 HR 0. 0 HR
2 CN 962 0. 0 HR 0. 0 HR 0. 0 HR
3 P138 0. 0 HR 0. 0 HR 0. 0 HR
4 Q i318 0. 0 HR 3. 2 HR 1. 6 HR
5 Zhongzi01 7. 8 HR 0. 0 HR 3. 9 HR
6 J inhuang 96B 0. 0 HR 8. 9 HR 4. 5 HR
7 Q i319 6. 9 HR 2. 4 HR 4. 7 HR
8 Pa405 15. 0 R 4. 6 HR 9. 8 HR
9 H an21 22. 6 R 14. 3 R 18. 5 R
10 Zhongzi03 0. 0 HR 31. 7 M R 16. 6 R
11 H an23 17. 6 R 16. 4 R 17. 0 R
12 X178 13. 6 R 21. 2 R 17. 4 R
13 H uobai 27. 5 R 15. 0 R 21. 3 R
14 K12 23. 8 R 23. 1 R 23. 5 R
15 H uangzao 4 15. 5 R 32. 4 M R 23. 9 R
16 H ai9221 31. 8 M R 34. 3 M R 33. 1 M R
17 CA 339 34. 5 M R 32. 2 M R 33. 4 M R
18 CA 156 34. 4 M R 31. 5 M R 32. 9 M R
19 Ye478 46. 4 S 42. 9 S 45. 2 S
20 Q i205 46. 4 S 46. 8 S 46. 6 S
21 Zhongzi451 44. 2 S 56. 3 S 50. 2 S
22 D huang212 60. 4 S 43. 6 S 52. 0 S
23 H 21 57. 8 S 49. 3 S 53. 6 S
24 W enhuang 53. 8 S 62. 4 S 58. 1 S
25 D an340 40. 2 S 77. 8 S 59. 0 S
26 Zong31 67. 1 S 54. 7 S 6019 S
27 CA 181 58. 8 S 65. 2 S 62. 0 S
28 5213 67. 1 S 60. 0 S 63. 5 S
29 Zi330 78. 3 S 50. 0 S 64. 2 S
30 Shen5003 71. 9 S 56. 9 S 64. 4 S
293 　　　作　　物　　学　　报 29 卷　

F ig. 1　D endrogram of 46 m aize inbred lines based on SSR m arkers
Table 1 (Con tinued)
N o. L ine
1999 2000 A verage overtwo years
D I% RE D I% RE D I% RE
31 H uang C (HC) 82. 4 S 50. 5 S 66. 5 S
32 T ie7922 82. 4 S 56. 5 S 69. 4 S
33 B73 69. 0 S 72. 9 S 7019 S
34 CA 091 95. 6 S 50. 0 S 72. 8 S
35 J i53 100. 0 S 50. 0 S 75. 0 S
36 4822 81. 2 S 72. 2 S 76. 7 S
37 B77 72. 1 S 83. 3 S 77. 7 S
38 U 8112 81. 4 S 75. 7 S 78. 6 S
39 M o17 98. 0 S 65. 0 S 81. 5 S
40 D anhuang 02 85. 9 S 80. 0 S 83. 0 S
41 Ye515 9019 S 75. 6 S 83. 3 S
42 374 100. 0 S 66. 7 S 83. 4 S
43 Ye107 87. 5 S 85. 4 S 86. 5 S
44 444 87. 5 S 89. 1 S 88. 3 S
45 7884 89. 4 S 91. 8 S 90. 6 S
46 E28 100. 0 S 96. 2 S 98. 1 S
　　RE: Resistance evaluation
112　F ield tests and artif ic ia l inocula tion
F ield tests w ere perfo rm ed at T angshan In st i2 tu te of A gricu ltu ra l Sciences, H ebei p rovince in1999 and 2000. T he inb reds w ere eva lua ted fo r re2act ion to SCM V as sing le2row p lo t in a com p lefeb lock design w ith tw o rep lica tes. T he p lo t w as 5mlong w ith 0. 76m spacing betw een row s. P lo ts w ereover2p lan ted and th inned to 18 p lan ts in each row.V iru s inocu la w ere p repared from infectedseed lings. L eaves w ith m o sa ic sym p tom w ere ho2m ogenated in 0. 01 m o löL pho sphate buffer (pH 7.0) in 1∶10 dilu t ion. P lan ts a t the 42 to 52 leafstage w ere rubbed w ith the inocu lum con ta in ingcarbo rundum tw ice w ith in a w eek in terva l. P lan tsw ere eva lua ted fo r viru s sym p tom s at w eek ly in ter2vals, beg inn ing a t 7210 days after the in it ia l inocu2la t ion.113　SSR pr im er selectionA ll 150 SSR p rim er pa irs selected from the p h i393　3 期 WAN G Zhen2H ua et a l. : Genetic D iversity of E lite M aize Germp lasm ⋯⋯

set w ere assayed u sing the sam p le of 15 typ ica l in2
b red lines. A fina l set of 49 p rim er pa irs g iven sta2
b le am p lif ica t ion p rofile w as cho sen fo r fu rther
ana lysis. D eta il info rm at ion regard ing each of the
49 p rim ers w as p resen ted in T ab le 2.
114　Am pl if ica tion and detection cond ition s
Genom ic DNA w as ex tracted from a bu lk of
ten p lan t leaves of each line u sing a m odified CTAB
p rocedu re [ 14 ]. T he PCR react ion s w ere perfo rm ed
u sing a PTC2200 T herm al Cycler (M J R esearch,
W atertow n, M A ). T he am p lif ica t ion p rogram w as
one cycle of in it ia l dena tu ra t ion a t 94℃ fo r 1 m in;
35 cycles of 94℃ fo r 1 m in, 60℃ fo r 2m in, and
72℃ fo r 2 m in; and one cycle of a fina l ex ten sion a t
72℃ fo r 5 m in. T he am p lif ied p roducts w ere kep t
a t 4℃ un t il electropho resis.
PCR react ion vo lum e w as 20ΛL con ta in ing
10mm o löL T ris2HC l, 50mm o löL KC l, 01001%
Gela t in, 215mm o löL M gC l2, 0116mm o löL each of
4dN T P, 10% glycero l, 0. 3Λm o löL SSR p rim er,
1U n it T aq DNA enzym e, and 50ng DNA tem p la te.
T he react ion m ix w as overla id w ith 28ΛL of m inera l
o il. A fter am p lif ica t ion, 3 - 4ΛL 5XSGB w ere
added to each tube. T he am p lif ica t ion p roducts
w ere separa ted by electropho resis in a M odel 16cm
×20cm ×011cm A T TO A E26220 vert ica l gel sys2
tem u sing 1×TBE on a 12% un2denatu red po ly2
acrylam ide gel w ith 28 lanes. 5 X174öH ae III u sed
as DNA ladder m arker w as loaded in to lane 1 per
com b. T he gels ran a t 250 con sisten t vo ltages fo r
215 o r 3h. A fter electropho resis, the gel w as sil2
ver2sta ined by the p rocedu re tha t rin sing by 10%
A cet ic A cid fo r 30 m in; qu ick rin sing by w ater fo r
th ree t im es; sta in ing by 0. 1% Silver N itra te fo r 30
m in; rin sing b riefly w ith w ater; develop ing by
215% N a2CO 3, and stopp ing by rin sing gels b riefly
w ith 3% N a22ED TA (o r 10% A cet ic A cid) , respec2
t ively. T he gel w as carefu lly slided on to a UV
tran sillum ina to r and pho tographed by Fo todyne
M P24 cam era w ith 20 cm ×26 cm hood and T ype
665 po lariod film.
115　Da ta ana lysis
T he ra t ing system w as taken on a sca le of 0
( sym p tom less) to 3 ( severe stun t ing w ith few ear
fo rm ed ) , and disease index (D I) (% ) w as u sed to
d ist ingu ish resistan t o r su scep t ib le geno types,
w h ich rep resen ted 0- 10 h igh resistan t (HR ) ; 1011
- 30 resistan t (R ) ; 30. 1 - 40 m odera te resistan t
(R ) , and > 40 su scep t ib le (S). D I= 2 (N o. of in2
fected p lan ts× ra ted sca le) ×100ö(To ta l p lan ts×
m ax im um scale)
F ragm en ts am p lif ied by SSR p rim ers w ere
sco red as p resen t (1) , ab sen t (0) o r m issing (9).
Genet ic sim ila rity (GS ) w as est im ated from the a l2
lele da ta u sing a sim p le m atch ing coefficien t such
tha t GS = m ö(m + n ) , w here m = the num ber of
m atches and n = the num ber of m ism atches[ 15 ].
C lu ster ana lysis of 46 m aize inb red lines w as per2
fo rm ed based on the m atrix of GS u sing U nw eigh t
Pa ir Group M ethod U sing A rithm etic A verages
(U PGM A ) clu stering a lgo rithm. T he GS m atrix
and clu ster w ere perfo rm ed w ith N T SYS2pc version
1. 8 softw are [ 16 ].
2　Results
211　Resistance of 46 ma ize inbred l ines to SCM V
M o st of elite inb red lines u sed cu rren t ly in
m aize b reed ing show ed h igh su scep t ib ility to SCM V
( T ab le 1 ) , such as M o17, Shen5003, Ye107,
Zi330, Ye478, D an340, T ie7922, U 8112, etc. O f
the 46 inb red lines tested, 8 (K22, CN 962, P138,
Q i318, Zhongzi01, J inhuang96B , Q i319, and
Pa405) w ere ra ted as h igh ly resistan t, 7 (H an21,
Zhongzi03, H an23, X178, H uobai, K12, and
H uangzao4) as resistan t, 3 (H ai9221, CA 339, and
CA 156) as m odera tely resistan t, and o thers as su s2
cep t ib le. T he react ion of 46 inb reds to SCM V w ere
genera lly con sisten t over tw o years.
212　Character iza tion of SSR markers
A to ta l of 49 SSR p rim ers from p h i set w ere
u sed to assay genet ic varia t ion am ong 46 inb red
lines, and p roduced 168 alleles ( T ab le 2 ). T he
num ber of a lleles per SSR locu s varied from 2 to
10, w ith an average of 3143. T he size of a lleles
ranged from 63bp to 336bp. M o st of 49 p rim ers de2
tected on ly one a llele per inb red line, w h ile severa l
p rim ers am p lif ied tw o bands in som e lines.
493 　　　作　　物　　学　　报 29 卷　

213　Genetic sim ilar it ies and cluster ana lysis of 46
inbred l ines
GS values (D ata un show n) am ong 46 lines cac2
u la ted based on 168 alleles ranged from 01562
betw een W enhuang vs. Zong31 up to 01928
betw een H uangzao4 vs. CN 962.
T he U PGM A algo rithm clu stered 46 inb red
lines in to severa l d ist inct group s based on 168 alle2
les, designa ted A to G (F ig. 1) , respect ively. C lu s2
ter A con ta in s H uangzao 4 and its deriva t ives,
w h ich is nam ed as Sip in tou group. C lu ster B con2
ta in s the lines from L uda R ed Cob group , such as
D an340 and E28. C lu ster C is of L ancaster group ,
such as M o17. B 73 and severa l inb reds from PN
germ p lasm are found in C lu ster D. C lu ster E con2
sists of Pa405 and H uobai, and clu ster F con ta in s
7884, J i53 and CA 339. C lu ster G con sists of tw o
subgroup s G I and G II. G I con ta in s five lines, i. e.
B 77, J inhuang 96B , Zi330, CA 091 and Zong31. G II
con ta in s six lines tha t a ll derived from PN
germ p lasm. Basica lly hetero t ic group ing of 46 in2
b red lines by SSR m arkers w as in agreem en t w ith
their know n pedigrees and b reeder’s experience.
O ne discrepancy w as tha t K22 and D an340 w ere
clu stered in to C lu ster B. K22 w as a conversion of
Ye478. Such incongru ity can be exp la ined by
severa l facto rs[ 17 ].
Table 2　　Chromosome loc i of 49 SSR pr imers, number of a lleles and size range detected among 46 l ines
Code SSR locus B in no. N o. alleles Size range (bp) Code SSR locus B in no. N o. alleles Size range (bp)
1 ph i056 1. 01 4 84- 93 26 ph i126 6. 00 10 134- 194
2 ph i097 1. 01 2 97- 100 27 ph i077 6. 01 8 124- 144
3 ph i037 1. 08 3 130- 158 28 ph i078 6. 05 2 122- 126
4 ph i011 1. 09 2 110- 122 29 ph i081 6. 05 3 160- 169
5 ph i055 1. 09 4 103- 115 30 ph i070 6. 07 2 73- 83
6 ph i064 1. 11 10 69- 113 31 ph i123 6. 07 2 143- 146
7 ph i120 1. 11 5 64- 88 32 ph i057 7. 01 3 145- 151
8 ph i083 2. 04 3 126- 134 33 ph i034 7. 02 4 120- 141
9 ph i090 2. 08 2 141- 151 34 ph i114 7. 02 5 135- 167
10 ph i127 2. 08 4 112- 128 35 ph i116 7. 06 4 151- 173
11 ph i029 3. 04 3 146- 164 36 ph i119 8. 02 3 162- 170
12 ph i053 3. 05 5 170- 194 37 ph i115 8. 03 3 93- 113
13 ph i073 3. 05 3 90- 99 38 ph i014 8. 04 2 157- 163
14 ph i046 3. 09 3 140- 152 39 ph i121 8. 04 2 99- 102
15 ph i072 4. 00 4 142- 162 40 ph i015 8. 08- 8. 09 4 82- 102
16 ph i074 4. 04 3 89- 95 41 ph i080 8. 09 5 140- 165
17 ph i096 4. 04 2 102- 112 42 ph i028 9. 01 3 63- 78
18 ph i086 4. 08 2 70- 73 43 ph i017 9. 02 3 101- 107
19 ph i092 4. 08 2 120- 128 44 ph i022 9. 02 3 124- 148
20 ph i019 4. 11 4 93- 102 45 ph i061 9. 03 2 80- 88
21 ph i113 5. 03- 5. 04 4 120- 336 46 ph i065 9. 03 3 132- 152
22 ph i048 5. 07 3 157- 169 47 ph i059 10. 02 2 146- 156
23 ph i058 5. 07 2 148- 151 48 ph i050 10. 03 3 80- 88
24 ph i085 5. 07 3 70- 90 49 ph i062 10. 04 2 161- 164
25 ph i128 5. 07 3 100- 110
　　C lu ster ana lysis show ed tha t there w ere a t
least fou r group s am ong 46 inb red lines, w h ich
have been found in Ch inese m aize germ p lasm [ 18 ] , i.
e. Singp ing tou (A ) , L uda R ed Cob (B ) , L ancaster
(C ) , and PA (D ). T he genet ic d istance ( GD )
betw een Sip ing tou and L uda R ed Cob group s is
clo ser, and bo th had sim ila r GD w ith group s L an2
caster and PA , respect ively. It is subgroup G II
tha t cou ld be m en t ioned as it con ta in s six inb red
lines tha t a ll derived from PN germ p lasm. G II
p robab ly can be nam ed as group PB com pared w ith
PA in w h ich the inb red lines a lso derived from PN
germ p lasm years ago. GD betw een PA and PB w as
rem arkab le based on SSR m arkers. It seem s tha t
PB is a d ist inct group tha t clearly d ifferen t ia tes
from PA and o ther group s. A ccu ra te genet ic rela2
t ion sh ip and hetero sis am ong PB , PA , L ancaster
and tw o dom est ic group s ( Sip ing tou, L uda R ed
593　3 期 WAN G Zhen2H ua et a l. : Genetic D iversity of E lite M aize Germp lasm ⋯⋯

Cob)w ou ld be invest iga ted u sing a large num ber of
inb red lines and m o re SSR m arkers.
3　D iscuss ion
U nder field condit ion s w ith art if icia l inocu la2
t ion, 8 and 7 inb red lines show h igh resistance and
resistance to SCM V , respect ively. How ever, 28
elite inb red lines u sed cu rren t ly in m aize b reed ing
are h igh su scep t ib ility, ind ica t ing tha t germ p lasm
basis fo r resistance to SCM V in m aize b reed ing
p rogram is narrow. Based on genet ic ana lysis, 46
inb red lines are clu stered in to 7 dist inct group s.
Group A con ta in s 2 resistan t lines, H uangzao 4
and CN 962, and Group B con ta in s 3 resistan t
lines, K22, H an 23 and H an21. Group E and sub2
group G II con ta in s 2 and 6 resistan t lines, respec2
t ively. Subgroup G I con ta in s 1 resistan t line,
J inghuang 96B.
R esu lts of the study are u sefu l fo r germ p lasm
im p rovem en t fo r resistance to SCM V based on
hetero t ic pa t tern. H uangzao 4 and CN 962 bo th
show a sign if ican t resistance to SCM V , w h ich
cou ld be u sed to im p rove W enhuang, D huang212,
444, H 21 and Ye515. K22, H an23 and H an21
show ing resistance to SCM V are in Group B , so
tha t these lines cou ld be u t ilized to im p rove
D an340, Zhongzi451, E28, 4822 and D anhuang02.
Group E and Group G II, w h ich con ta in a la rge
num ber of resistan t germ p lasm , po ssess a h igh po2
ten t ia l u t iliza t ion in im p rovem en t of resistance to
SCM V. How ever, no resistan t inb red lines can be
found in Group s C, D and F.
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693 　　　作　　物　　学　　报 29 卷