---

作 者 ：彭玉兰 胡运乾 孙航

期 刊 ：云南植物研究 2003年 25卷 05期 页码：1-3

关键词：栌菊木属；等位酶；生物地理；保护生物学；

Keywords:Leymus,

摘 要 ：对我国西南特有的菊科单种属植物栌菊木10个居群、149个个体、11个酶系统及16个酶位点的水平淀粉凝胶电泳分析表明，栌菊木的遗传多样性水平在特有种中较高，在居群水平上，平均每个位点的等位基因数A=11～14，多态位点百分数P=63%～438%，实际杂合度Ho=0063～0250，期望杂合度He=0043～0194；物种水平上A=16，P=375%，Ho=0143，He=0141。居群间遗传一致度I=0902～1000，杂合性基因多样度比率FST为02395。栌菊木居群间分化程度较大，云南南盘江流域碧云寺居群遗传多样性较低，明显低于金沙江流域的居群。栌菊木可能是来自冈瓦纳古陆祖先的后裔，可能是古地中海退却以后在金沙江干热河谷分化出来的特有属，并且可能由于湿度等生态因子的限制，其分布区未能进一步扩大，仅在南盘江流域形成零散分布。等位酶分析结果还表明栌菊木遗传多样性总体水平较高，建议对遗传多样性较高的金沙江流域的居群加以保护。

Abstract:The gliadin genetic polymorphism among 45 accessions of 20 species and 1 subspecies in Leymus Hochst.were analyzed by using acid polyacrylamide gel electrophoresis (A-PAGE).The results showed as follows:(1) 43 types of gliadin patterns were obtained from the tested materials and 41 materials had their unique patterns respectively.A total of 38 gliadin bands were separated by electrophoresis,of all showing polymorphism,which amounts to 100%.(2) Distinct gliadin genetic variations were presented among the populations.The gliadin genetic variations among different species were more abundant than that of different accessions,and it revealed that the gliadin patterns can be used as a fingerprint to survey different materials in Leymus Hochst.(3) The relationships among species in Leymus Hochst.according to gliadin data are basically comparable with that obtained from morphological data,so that the gliadin patterns data can be used to investigate genetic variation and biosystematic relationships among species in Leymus Hochst.

---