Abstract:Eight steroidal compounds were isolated from the rhizomes of Aspidistra elatior collected form Yongshan County of Yunnan Province,China. AMong them ,six known compounds were identified as (25S)-3β-hydroxy-spirost-5-ene 3-O-β-D-glucopyranosyl(1→)[β-D-xylopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside(1),(25S)-26-O-β-D-glucopyranosyl-furost-5-en-3β,22ζ,26-triol3-O-β-D-glucopyranosyl(1→)[β-D-xylopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside(2), tupiloside H (3), neopentrogenin 5-O-β-D-glucopyranoside(5), tupstroside G(6)and neopentrogenin (7).