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作 者 ：周颂东，罗鹏

期 刊 ：云南植物研究 2002年 24卷 04期 页码：1-3

关键词：播娘蒿；下胚轴；再生植株；影响因素；

Keywords:Descurainia sophia,

摘 要 ：以播娘蒿(Descurainia sophia)下胚轴为外植体，研究影响其分化的若干因素。结果表明：用附加2，4-D的培养基，对下胚轴切段预培养5d可提高分化频率；1.7mg/L AgNO3对分化出芽有很大促进作用，最佳激素组合为2.0mg/L 6-BA+0.2mg/L NAA；黄化苗的下胚轴比绿苗的下胚轴对激素反应更敏感，最高分化频率可达86.05%。再生芽转至1/2 MS+0.5mg/L NAA+0.5mg/L IBA的培养基中，生根率100%。

Abstract:The hypocotyl of D.sophia was cultured to study the effect of some factors on the rate of bud regeneration.The results showed that the rate of hypocotyl differentiating was improved via a preculture of five-day period with the medium containing 2,4-D;AgNO3(1.7mg/L)could greatly improve the differentiating rate of hypocotyl;the best hormone combination wqs 2.0mg/L6-BA+0.2mg/L NAA when 1.7mg/L AgNO3 was added to this medium; the hypocotyl of yillow seedlings was more sensitive to hormone than that of green seedlings,its differentiating rate could reach as high as 86.05%.When the regeneration buds were transferred 1/2 MS+0.5mg/L NAA+0.5mg/L IBA,the rate of regeneration roots reached 100%

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