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作 者 ：王东；季本仁；曾英；余兵

期 刊 ：云南植物研究 1996年 18卷 04期 页码：1-3

关键词：海芋；胰蛋白酶抑制剂；分离与纯化；

Keywords:Alocasia macrorrhiza,

摘 要 ：利用亲和层析和分子筛凝胶过滤等技术，从海芋（Alocasia macrorrhiza）根茎中分离纯化到一种胰蛋白酶抑制剂，简称 AMTI。经 PAGE、SDS－PAGE和 Western blot鉴定均显示单一条带，经SDS－PAGE测定，其分子量为 22 000，经等电聚焦（IEF）测定，其等电点为 6.2。根据对胰蛋白酶的抑制比可知该抑制剂为单头抑制剂，其抑制活性在 60℃和 pH5~11范围内保持稳定。

Abstract:A trypsin inhibitor named AMTI has been isolated and purified from the rhizome of Alocasia macrorrhiza by a procedure including affinity chromatography on trypsina- Sepharose 4B and gel filtration with Sephadex G-100. This inhibitor was homogeneous on PAGE, SDS-PAGE, IEF and Western blot. It appeared as a single band of about 22 000 dalton on SDS-PAGE. The isoelectric fo- cusing(IEF) showed that its PI was about 6.2. lts inhibitory activity to trypsin indicated that AMTI was a single headed inhibitor. This inhibitor was stable against heat and pH.

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