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作 者 ：张伟媚 程在全 陈善娜

期 刊 ：云南植物研究 2004年 26卷 01期 页码：1-3

关键词：Bt基因；水稻愈伤组织；根癌农杆菌；基因转化；PCR分析；

Keywords:Trigonobalanus doichangensis；RAPD；Genetic diversity；Population genetic structure；Conservation,

摘 要 ：通过农杆菌介导法，将Bt毒蛋白基因的一种—cryIAb基因导入2个云南水稻栽培品种(滇系4号，合系39号)的愈伤组织中，经过潮霉素筛选后，获得了一批抗性苗，部分苗经PCR检测为阳性，GUS组织化学染色分析发现Bt基因已整合到水稻基因组，并由此建立了一套有效的水稻遗传转化体系。实验中发现培养器皿的透气性对水稻愈伤组织的形成、生长具有较大的影响，提高培养基的渗透势能够提高愈伤组织的分化率。选择合适的洗菌液渗透势能够大大降低洗菌对愈伤组织所造成的损伤，从而提高转化率。

Abstract:The genetic diversity and population genetic structure of five populations with total of 99 Trigonobalanus doichangensis individuals were estimated with random amplified polymorphic DNA (RAPD) markers.One hundred and fiftyseven loci were amplified with 16 oligonucleotide primers,of which 83 loci (5287％) were polymorphic.Shannon‘s information index (I) and Nei‘s gene diversity (h) were 02431 and 01595 respectively at the species level.Total genetic diversity (Ht) was 01600,and the genetic diversity within populations (Hs) was 00749.The genetic variation between different populations accounted for 5320％ of the total.This indicated that genetic variation existed mainly between populations rather than within populations.In comparison with other species from Fagaceae,the genetic diversity of Tdoichangensis is lower and the gene flow among populations is very restricted.These may be attributed to the changes of environment during the species evolution,habitat destruction and excessively making use of the species resources by the people.Based on the research results,the conservation strategies of genetic diversity for the species were put forward.

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