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期 刊 ：植物遗传资源学报 2015年 16卷 3期

关键词：猕猴桃；雄株；ISSR；聚类分析；遗传多样性；

Keywords:Kiwifruit, Male cultivars, ISSR, Cluster analysis, Genetic diversity,

摘 要 ：利用ISSR分子标记对雄性猕猴桃16个材料进行遗传多样性分析。从100条引物中筛选出10条引物用于ISSR扩增，共扩增出172条带，其中多态性条带140条，多态性百分率为81.4％；经POPGENE 1.32软件分析结果显示，16个雄性猕猴桃材料的遗传距离在0.15～0.51之间，平均Nei′s 基因多样性指数（H）为0.24，平均Shannon信息指数(I)为0.40；聚类分析结果显示，在遗传相似系数为0.64处可将供试材料分成4类，第Ⅰ类为中华和美味，第Ⅱ类为阔叶、毛花，第Ⅲ类为魁绿，第Ⅳ类为四萼。结果表明ISSR可用于雄性猕猴桃遗传多样性研究，该研究结果可为猕猴桃种质资源的进一步开发利用提供重要信息。

Abstract:In this study, 16 male kiwifruit cultivars were analyzed using inter-simple sequence repeat markers (ISSR) to invettigate the genetic diversity. 10 primers selected from 100 primers were used for ISSR amplification. A total of 172 bands were generated, of which 140 bands were polymorphic bands(the percentage of polymorphic band, PPB=81.4%). The results of POPGENE version 1.32 analysis showed that the genetic distance of the 16 male kiwifruit cultivars was between 0.15-0.51 and the genetic diversity index (H) and Shannon index (I) was 0.24, 0.40, respectively. These cultivars were divided into 4 groups at the genetic similarity of 0.64. Class Ⅰ was made up of A. chinensis and A. deliciosa. Class Ⅱ was A. latifolia and A. eriantha. Class Ⅲ was ‘Kuilv‘ and Class Ⅳ was A. tetramera. It is indicated that ISSR markers were feasible to analyze the genetic diversity of male kiwifruits and the results would facilitate the explotation and utilization of kiwifruits.

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