Abstract:Some sori of Adiantum capillus-veneris were used. Some sori were collected the fern leaves. Sporangia were sown on the surface of MS mediuln with 6--BA or without hormone, the spores germinating percentage was 20--25%. when adding GA,, the spores germinating percentage was 75-95%. Spores after germina- ting and become protonemae, the protonemae were transferred on the potato medium adding with NAA or 2, 4 -D and the MS medium adding witll GA3, NAA+ Homicacid-Na, MS medium adding with CM+ BA+ NAA. These results of culture show that MS medium adding with GA, should be nice for spore germina-tionl potato medium adding with NAA or 2, 4 -D should be efficient for sporo- phyte formation. In our experiments spent six to nine monthes from spore germina- tion, at first, developes as a filamentous protonema, then becomcs sporophyte.