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期 刊 ：植物遗传资源学报 2015年 16卷 1期

关键词：连翘属；染色体；核型参数；亲缘关系；

Keywords:Forythia, Chromosome, Karyotye analysis, Genetic relationship,

摘 要 ：摘要：采用常规压片法对4个连翘属原种以及6个金钟连翘品种和2个朝鲜连翘品种的核型进行了分析。研究结果发现，除金钟连翘品种 ‘Goldrausch’为四倍体（2n=4x=56）外，其余11份种质均为二倍体（2n=2x=28），染色体基数为14。连翘、卵叶连翘、东北连翘、朝鲜连翘的核型分别为2A、3B、2B、3B型，6个金钟连翘品种的核型包括2A、2B、3B 3种，2个朝鲜连翘品种的核型包括2B、3B。12份连翘种质的着丝粒指数差异性不大，介于9.65~50.00%，染色体类型以中部（m）、近中部（sm）以及近端部（st）染色体为主，未发现端部染色体和随体。结果表明，连翘和东北连翘的亲缘关系最近，与卵叶连翘的亲缘关系最远，金钟连翘品种与东北连翘、朝鲜连翘之间亲缘关系较近。研究结果为连翘属种间、品种间杂交育种和亲缘关系研究提供了细胞学基础。

Abstract:Abstract：The chromosome numbers and karyotypes of 12 Forsythia species and cultivars were studied using squashing method, and the results showed that 11 other Forsythia species and cultivars were diploid (2n=2x=28), except F. intermedia ‘Goldrausch’, which was tetraloid (2n=4x=56). The karyotypes of F. suspensa, F. ovate, F. mandshurica and F. koreana were 2A, 3B, 2B, 3B, separately. The karyotypes of 6 F. intermedia cultivars included 2A, 2B and 3B. The karyotypes of 2 F. koreana cultivars included 2B and 3B. Generally, the centromere index of 12 Forsythia species and cultivars were not distinct different, which arranged from 9.65% to 50.00%. The karyotypes of all the tested Forsythia taxa were mainly made of m, sm, and st, without chromosome or satellite The results from clustering analysis on karyotype parameters of the 4 Forsythia species showed that F. suspense and F. mandshurica have near genetic relationship, while the genetic relationship between F. suspense and F. ovata was distant. Meanwhile, F. intermedia cultiawrs have nearer genetic relationship with F. mandshurica and F. koreana, than F. suspense and F. ovata. The results provided cytological information for genetic relationship analysis and parent selection in interspecific hybridization in Forsythia genus.

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