Abstract:The essential oil of roots from Valeriana iatamansi Jones was analysedqualitatively and quantitatively by means of fractional distillation, silican gel or alumina column chromatography, TLC, GLC, chemical and spectrometricmethods. Eight components have been identified,limonene (0. 8%). l, 8-cineole (0. 3%), Plcymene (1 .2 %), borneol acetate (7. 2%), borneol, (4. 5%), nerolidol (1.0%), maaliol(11.6%).