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期 刊 ：植物遗传资源学报 2014年 15卷 3期

关键词：河北省；冬小麦；品种；SSR标记；遗传多样性；

Keywords:Winter wheat, Cultivars, SSR marker, Hebei province, Genetic diversity,

摘 要 ：利用79对多态性较高的SSR引物，对河北省1997-2007年间审定的冬小麦品种及国家小麦区试抗旱对照品种晋麦47和洛旱2号，共计87个冬小麦品种进行遗传多样性分析。79对SSR引物共检测出175个等位变异位点，每对引物可产生1~6条等位变异位点，平均2.215条。标记位点多态性信息含量（PIC）变幅为0.824~0.998，平均为0.941；有效等位基因数（Ne）变幅为1.644~20.333，平均4.708；香农指数（H’）变幅为0.148~1.102，平均为0.544，说明河北省冬小麦品种SSR遗传多样性较低。品种间遗传相似系数（GS）变幅为0.184~0.899，平均为0.418，其中河农826与石家庄8号间的遗传相似性最高，GS高达0.899，71-3与藁优9618间的遗传相似性最低，GS为0.184。不同育种单位培育的小麦品种平均遗传相似系数存在较大差异。UPGMA遗传相似性聚类表明，石家庄市小麦新品种新技术研究所培育的小麦品种与其他单位品种存在较大的遗传差异。

Abstract:Seventy-nine SSR primers with high polymorphisms and abundant information were used to detect genetic diversity of the total 87 winter wheat cultivars, including 85 cultivars authorized in Hebei province, and the two control cultivars used in the regional test of China for drought-resistant wheat variety, Jinmai 47 and Luohan 2. A total of 175 allelic variation loci were detected in 79 SSR primers, ranging from 1 to 6 per primer pair with an average of 2.215. The polymorphism information content (PIC) ranged from 0.824 to 0.998, with an average of 0.941. The effective number of alleles for each locus (Ne) varied from 1.644 to 20.333, with an average of 4.708. The Shannon-weaver diversity index, also referred to as Shannon-Weiner index (H’) ranged from 0.148 to 1.102 and averaged 0.544. These indicated that genetic diversity of 87 tested cultivars was low based on SSR markers. The genetic similarity coefficients (GS) varied from 0.184 to 0.899, with an average of 0.418. Among them, the GS (0.899) between Henong 826 and Shijiazhuang 8 was the highest, but the GS (0.184) between 71-3 and Gaoyou 9618 is the lowest. The GS averages were different among the cultivars released from different breeders. The UPGMA cluster indicated that genetic difference was high between the cultivars from Shijiazhuang New Wheat Variety and Technology Research Institute and those from other breeders.

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