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作 者 ：刘成运

期 刊 ：云南植物研究 1984年 6卷 04期 页码：1-3

关键词：山茶雄配子体发育；三磷酸腺苷酶；酸性磷酸酶细胞；化学定位；云南；

Keywords:cytochemical, localization, triphosphatase, camellia,

摘 要 ：人工培养的云南山茶（Camellia reticulata Lindl.）雌配子体发育的不同时期，在花粉管,营养核及生殖细胞内二磷酸腺苷酶和酸性磷酸酶的相对活性各不相同。萌发初期，花粉粒内三磷酸腺苷酶的反应特别地强烈，而酸仕磷酸酶的活性仅显示在花粉粒内壁及萌发孔周围。当萌发后，在花粉管内强烈地显示了这两种酶的活性，特别是顶端生长区。当营养核及生殖细胞进入花粉管后，酶的活性随它们在管内向端运动的速度而变化。在贴合期，在二者接触膜上显示出较明显的酶的活性。精子形成以后，营养核酶的活性降低。

Abstract:The relative activity of ATPase, β-GPase and P-NPPase were different in the pollen tube, vegetative nucleus and generative cell at various stages of devcloping gametophyte of Camellia reticulata Lindl. in vitro. The ATPase activiffy was particularly intense in the pollen grain, while the activity of β-GPase and P-NPPase were only localized on the intine and around the germinal aperture at the initial stage of the germination. After the germination, the activity of both enzymes were show intensely in the tube and top growth region in particular.

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