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期 刊 ：植物遗传资源学报 2016年 17卷 4期

关键词：EST-SSR分子标记；以礼草属；遗传多样性；聚类分析；

Keywords:EST-SSR Maker, KengyiliaYen, genetic diversity, cluster analysis,

摘 要 ：利用EST-SSR分子标记技术对青海高原以礼草属3个物种的21份材料进行遗传相关分析。在遗传多样性分析中发现97对引物中共有60对引物扩增出103条条带，各个引物的扩增条带数从1到5，每个位点的平均等位变异数1.70。经过Popgene pc软件分析，梭罗草的遗传多样性水平最高[PP=62.14%，h=0.2345],糙毛以礼草次之[PP=58.25%，h=0.2211]，大颖草最低[PP=44.66 %，h=0.1687]。在遗传相似性分析中发现梭罗草和大颖草的遗传距离最小，相似性程度最高，糙毛以礼草与梭罗草和大颖草的遗传距离都较远。其中梭罗草居群中1号居群与其他居群遗传距离相对较远，糙毛以礼草居群中14号材料与其他居群遗传距离相对较远，大颖草居群中18号居群与其他居群遗传距离相对较远。利用NTSYS2.10e聚类分析表明21个居群可分为三个组群，梭罗草居群10个；糙毛以礼草居群7个；大颖草居群4个。本研究可为拓宽小麦族植物的遗传基础及物种合理布局提供科学参考。

Abstract:The genetic diversity of 21 populations of 3 varieties of KengyiliaYen was investigated by EST-SSR markers. There are 103 polymorphic bands were created by 60 avaliableEST-SSR primers which were selected from 97 primers in those materials. Every primers amplifying 1 to 5 bands ,and every band have an average number of alleles of 1.70. The genetic diversity of Kengyilia thoroldiana was the highest and that Kengyilia hirsute was the second highest. Kengyilia grandiglumis with the lowest PP of 44.66% in genetic diversity. The distance between Kengyilia thoroldiana and Kengyilia grandiglumis of genetic similarity analysis was minimal and the genetic similarity was maximum. The genetic distance of Kengyilia hirsute and Kengyilia grandiglumis and Kengyilia thoroldiana was far. The number 1 population of Kengyilia thoroldiana was far compare with other populations in genetic distance. The number 14 population of Kengyilia hirsute was far compare with other populations in genetic distance. The number 18 population of Kengyilia grandiglumis was far compare with other populations in genetic distance.The 21 populations was classified 3 groups :10 Kengyilia thoroldiana populations,7 Kengyilia hirsute populations and 4 Kengyilia grandiglumis populations by NTSYS 2.10e. Resonable reference can be provided to further research of genetic resource in Triticeae in this research.

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